Youtube daily report w Dec 30 2017

Snickerstream is now back from the dead. You can stream your 3DS screen easily with this app

All you have to do is install custom firmware on your console then run BootNTR and you are ready to go.

This is my New 3DS XL.

And it is running custom firmware on SysNAND.

Now we need to install BootNTR. We will use FBI to install it. Make sure your console is connected to the internet.

Choose TitleDB. And then search for BootNTR Selector

Then install it

Now press any button and press B to return to the main menu then choose Remote Install

Choose Receive URLs over the network

This is your console's IP address and port number. Make a note of this.

Press B twice then press Start to exit FBI

Now run BootNTR Selector

Open the web browser and go to this URL

You can read what Snickerstream is from this page

Click the releases tab and download the latest version of Snickerstream

You can choose between 32bit or 64bit version.

Extract the package anywhere on your computer

Then run it

Enter the console's IP address then press Connect.

If done correctly, you will see your 3DS screen on your computer

You can press Up or Down to scale up or scale down the screen.

But you will start noticing some frame rate lags after scaling up the screen

Let's try playing a game

Too bad, you cannot use input redirection with this app for now.

This app is intended for streaming purpose only

Thank you for watching this short tutorial. I hope you liked it. Please subscribe for more upcoming videos

For more infomation >> 3DS Tutorial : How to stream 3DS Screen to Computer using Sneakerstream - Duration: 3:48.

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НОВОГОДНИЙ РЕЙД | Cam Pranks — Пранки c камерами - Duration: 8:50.

For more infomation >> НОВОГОДНИЙ РЕЙД | Cam Pranks — Пранки c камерами - Duration: 8:50.

-------------------------------------------

Different Betwin DIAL-UP VS BROADBAND.All about DSL,CABIL INTERNET,SATELLITE,FIBER OPTIC - Duration: 7:19.

hi friend tech samon channel ea apnader swagato,tech samon channel theke ami samrat.

For more infomation >> Different Betwin DIAL-UP VS BROADBAND.All about DSL,CABIL INTERNET,SATELLITE,FIBER OPTIC - Duration: 7:19.

-------------------------------------------

PREGUNTAS Y RESPUESTAS | Mateo Sánchez - Duration: 2:46.

For more infomation >> PREGUNTAS Y RESPUESTAS | Mateo Sánchez - Duration: 2:46.

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Un CHEVAL dans une salle de bain ???!!! / CSO #46 - Duration: 1:57.

For more infomation >> Un CHEVAL dans une salle de bain ???!!! / CSO #46 - Duration: 1:57.

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The future of shipping

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Capodanno in diretta da Maratea con Albano e Romina Power | K.N.B.T - Duration: 6:26.

For more infomation >> Capodanno in diretta da Maratea con Albano e Romina Power | K.N.B.T - Duration: 6:26.

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Uomini e Donne, Anna Tedesco lascia il programma? | M.C.G.S - Duration: 4:01.

For more infomation >> Uomini e Donne, Anna Tedesco lascia il programma? | M.C.G.S - Duration: 4:01.

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Uomini e Donne over: Gemma ed i suoi regali dall'Oriente per Giorgio Manetti | Wind Zuiden - Duration: 3:26.

For more infomation >> Uomini e Donne over: Gemma ed i suoi regali dall'Oriente per Giorgio Manetti | Wind Zuiden - Duration: 3:26.

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Romina power Al Bano Carrisi è molto deluso e arrabbiato | WInd Zuiden - Duration: 3:32.

For more infomation >> Romina power Al Bano Carrisi è molto deluso e arrabbiato | WInd Zuiden - Duration: 3:32.

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Flavio Briatore e le frasi infelici: "non so come si possa vivere con 1300 euro al mese." - Duration: 9:13.

For more infomation >> Flavio Briatore e le frasi infelici: "non so come si possa vivere con 1300 euro al mese." - Duration: 9:13.

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Renault Mégane Scénic 1.6E RN - Duration: 0:43.

For more infomation >> Renault Mégane Scénic 1.6E RN - Duration: 0:43.

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Quiet Time | Train Simulator 2018 | Stamford Trash | GE E-33 NH EF-4 | New York to New Haven - Duration: 1:04:30.

For more infomation >> Quiet Time | Train Simulator 2018 | Stamford Trash | GE E-33 NH EF-4 | New York to New Haven - Duration: 1:04:30.

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Citroën Grand C4 Picasso 1.6 e-THP 7pl Intesive 1e eigenaar orig.nl geleverd RIJKLAARPRIJS - Duration: 1:01.

For more infomation >> Citroën Grand C4 Picasso 1.6 e-THP 7pl Intesive 1e eigenaar orig.nl geleverd RIJKLAARPRIJS - Duration: 1:01.

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Mercedes-Benz E-Klasse 320 AVANTGARDE A4, AUTOMAAT, SEDAN! - Duration: 1:00.

For more infomation >> Mercedes-Benz E-Klasse 320 AVANTGARDE A4, AUTOMAAT, SEDAN! - Duration: 1:00.

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Peugeot 108 1.0 e-VTi Blue Lease / 5-DRS / AIRCO / WEINIG KM - Duration: 0:57.

For more infomation >> Peugeot 108 1.0 e-VTi Blue Lease / 5-DRS / AIRCO / WEINIG KM - Duration: 0:57.

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Mercedes-Benz E-Klasse Coupé 350 4Matic 306PK Prestige 7G-Tronic Plus AMG Pakket (51.023Km Sportled - Duration: 0:57.

For more infomation >> Mercedes-Benz E-Klasse Coupé 350 4Matic 306PK Prestige 7G-Tronic Plus AMG Pakket (51.023Km Sportled - Duration: 0:57.

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Citroën C3 1.6 e-HDi Selection - Duration: 0:59.

For more infomation >> Citroën C3 1.6 e-HDi Selection - Duration: 0:59.

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Citroën C1 1.0 e-VTi Feel 5-DRS. (Airco/Bluetooth) - Duration: 0:52.

For more infomation >> Citroën C1 1.0 e-VTi Feel 5-DRS. (Airco/Bluetooth) - Duration: 0:52.

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Uomini e Donne news, Cristian e Tara si sono separati? Ecco la foto della verità - Duration: 4:22.

For more infomation >> Uomini e Donne news, Cristian e Tara si sono separati? Ecco la foto della verità - Duration: 4:22.

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UeD, Cosa ha regalato Tina Cipollari a Gemma per questo Natale? | Wind Zuiden - Duration: 3:21.

For more infomation >> UeD, Cosa ha regalato Tina Cipollari a Gemma per questo Natale? | Wind Zuiden - Duration: 3:21.

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Gossip, Cecilia Rodriguez: 'Ignazio Moser non mi sopporta più' | Wind Zuiden - Duration: 3:32.

For more infomation >> Gossip, Cecilia Rodriguez: 'Ignazio Moser non mi sopporta più' | Wind Zuiden - Duration: 3:32.

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The Last Jedi psychology explained - Duration: 7:20.

Too many jokes!

Too many surprises!

Too many porgs!

There, now you don't need to write that in the comments section.

Let's move on to discussing how The Last Jedi is the most psychologically complex Star

Wars film ever made.

Star Wars has a lot to say about emotions.

The original trilogy encourages us to feel our emotions, quiet our minds, focus on the

present moment.

This is called mindfulness.

We see it when when Luke destroys the Death Star, during Yoda's Jedi training, and it's

what helps Darth Vader turn against the Emperor.

The prequel trilogy warns us about the dangers of avoiding emotions.

This is called experiential avoidance.

Anakin never wants to experience the pain of losing someone he loves ever again.

This is why he becomes obsessed with trying to protect Padme and it's what leads him

to the dark side.

The Force Awakens is mostly about new characters set against familiar Star Wars ideas.

It doesn't say anything new about emotions.

We see Rey use mindfulness and Kylo loses his temper, just like his grandfather and

a lot of people who struggle with experiential avoidance.

It does has some new ideas about heroism, but nothing about emotions.

The Last Jedi goes into a very different direction.

It's a story that explores the oldest, most universal emotion -- fear.

To explain how, I have to get into spoilers.

If you haven't watched The Last Jedi, pause this video, go see it because it's awesome,

and then come back here.

Star Wars has discussed fear before, but it did it badly.

In The Phantom Menace, Yoda says "Fear leads to anger, anger leads to hate, hate…leads

to suffering."

Fear doesn't necessarily lead to anger or suffering.

Fear is an emotion that is activated when we sense danger.

It transformers your body to run faster, jump higher, and fight harder.

It also focuses all of your attention on your immediate threat.

For a long time researchers have known fear leads to fight or flight - a drive to face

danger head on or run away to safety.

We see this a lot in The Last Jedi - Poe's instinct is to attack when threatened.

Finn is afraid of being captured by the First Order and tries to leave the resistance on

an escape pod.

Luke is afraid of who he has become and is hiding at the first Jedi temple on Ahch-To.

While fight and flight has dominated fear research, in 2000 psychologist Shelly Taylor

introduced a new idea: Tend and befriend.

Tending means taking care of yourself, those around you, and helping people to feel calm.

Befriending is about connecting with other people to make your community safer.

We see a lot of this in The Last Jedi too - Leia is focused on protecting the resistance

and connecting with allies.

Rey tries to help Luke and Kylo in the hopes that they will join the resistance.

Rose saves Finn's life and tells him, "We're going to win this war not by fighting what

we hate, but saving what we love."

We've never seen such a detailed exploration of fear before in Star Wars and we rarely

see it in Hollywood.

While everyone is capable of both, men tend to rely on fight or flight strategies while

women are more likely to tend and befriend.

Since most films are created by men, stories tend to glorify fight or flight while tend

and befriend is an secondary response at best and a cowardly act at worst.

The Last Jedi breaks this trend by showing us both approaches are equally important to

survival.

That's why the most awesome moment in this film belongs to Vice Admiral Holdo.

She is the integration of fight or flight and tend and befriend.

She spends the whole movie protecting the resistance but attacks when it's the only

way for the resistance to survive.

The Last Jedi also shows us what happens when we're haunted by our fears.

Luke sensed a powerful darkness growing in Ben Solo and for one brief moment activated

his lightsaber with the intention of killing Ben.

Ben destroyed Luke's Jedi temple, killed many of its students, and transformed into

Kylo Ren.

Luke is traumatized by this.

He runs away to Ahch-To, closes himself off to The Force, and decides the Jedi order must

die with him.

Luke's story is an honest representation of trauma.

It's not seeing something horrific that makes an event traumatizing.

Luke's seen plenty of people die before and he's killed a lot of people too.

Traumas happen when an event challenges your fundamental beliefs.

Luke was taught the Jedi are the guardians of peace and justice but now as a Jedi he's

contributed to his nephew's turn to the dark side and the rise of the First Order.

When we avoid talking about trauma, as Luke has, we get stuck.

The normal healing that's supposed to happen never does.

Luke is stuck thinking about his guilt letting down Han and Leia, he's disgusted with Jedi

teachings, and ashamed about what he's become.

The only way to move forward after a trauma is by finding a way to integrate the experience

into your life.

This means talking about what happened, re-examining your role in the event, and revising your

beliefs to accurately reflect the trauma.

This is what Rey and Yoda help Luke to do, realize that he can learn from his failure

and help those who are in danger.

That's why the second most awesome moment in this film is seeing Luke confront his trauma

through a peaceful nonviolent sacrifice.

The Last Jedi defies our expectations and challenges our fundamental beliefs about Star

Wars, heroes, and villains.

I know a lot of fans are struggling with what happens to Luke Skywalker.

The original trilogy is a mythical heroes journey about Luke's call to adventure,

transformation into a Jedi, and restoring peace to the galaxy.

That's how myths work: heroes always do good and they defeat evil.

But myths aren't real.

Sometimes heroes make mistakes and have to live with the consequences.

The Last Jedi is an honest account of what happens to real life heroes, what it means

to be brave in the face of fear, and the consequences of facing traumatic situations.

Like Luke, Star Wars was stuck in myth.

But you can't renew Star Wars by repeating mythical formulas.

By embracing reality, Star Wars has grown beyond what it was and is reborn into something

completely new.

What did you think of The Last Jedi?

Let me know in the comments below.

There's one more way we can react to fear - we can freeze.

We see that when Kylo Ren intends to kill his mom but freezes when the moment comes.

I didn't talk about that earlier because this video is already longer than what I intended

and I couldn't find a way to easily squeeze it in so I'm putting it here.

For more infomation >> The Last Jedi psychology explained - Duration: 7:20.

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Ogni vita dovrebbe essere miracolosa (Everyone's Life Should Be Miraculous) - Duration: 28:19.

For more infomation >> Ogni vita dovrebbe essere miracolosa (Everyone's Life Should Be Miraculous) - Duration: 28:19.

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Seat Leon 2.0 TDI Businessline Automaat Navi Elekt Pakket 159.000 KM - Duration: 1:27.

For more infomation >> Seat Leon 2.0 TDI Businessline Automaat Navi Elekt Pakket 159.000 KM - Duration: 1:27.

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王大治升级当爸爸，网友：他和董洁什么时候结束的？ - Duration: 11:49.

For more infomation >> 王大治升级当爸爸，网友：他和董洁什么时候结束的？ - Duration: 11:49.

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Family fun on the water

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Renault Captur 90pk TCe Dynamique | Camera | Climate Controle | R-LINK | Cruise Controle | Metallic - Duration: 0:54.

For more infomation >> Renault Captur 90pk TCe Dynamique | Camera | Climate Controle | R-LINK | Cruise Controle | Metallic - Duration: 0:54.

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Volvo V40 T2 122PK R-Design - Duration: 0:59.

For more infomation >> Volvo V40 T2 122PK R-Design - Duration: 0:59.

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3DS Tutorial : How to stream 3DS Screen to Computer using Sneakerstream - Duration: 3:48.

Snickerstream is now back from the dead. You can stream your 3DS screen easily with this app

All you have to do is install custom firmware on your console then run BootNTR and you are ready to go.

This is my New 3DS XL.

And it is running custom firmware on SysNAND.

Now we need to install BootNTR. We will use FBI to install it. Make sure your console is connected to the internet.

Choose TitleDB. And then search for BootNTR Selector

Then install it

Now press any button and press B to return to the main menu then choose Remote Install

Choose Receive URLs over the network

This is your console's IP address and port number. Make a note of this.

Press B twice then press Start to exit FBI

Now run BootNTR Selector

Open the web browser and go to this URL

You can read what Snickerstream is from this page

Click the releases tab and download the latest version of Snickerstream

You can choose between 32bit or 64bit version.

Extract the package anywhere on your computer

Then run it

Enter the console's IP address then press Connect.

If done correctly, you will see your 3DS screen on your computer

You can press Up or Down to scale up or scale down the screen.

But you will start noticing some frame rate lags after scaling up the screen

Let's try playing a game

Too bad, you cannot use input redirection with this app for now.

This app is intended for streaming purpose only

Thank you for watching this short tutorial. I hope you liked it. Please subscribe for more upcoming videos

For more infomation >> 3DS Tutorial : How to stream 3DS Screen to Computer using Sneakerstream - Duration: 3:48.

-------------------------------------------

НОВОГОДНИЙ РЕЙД | Cam Pranks — Пранки c камерами - Duration: 8:50.

For more infomation >> НОВОГОДНИЙ РЕЙД | Cam Pranks — Пранки c камерами - Duration: 8:50.

-------------------------------------------

Different Betwin DIAL-UP VS BROADBAND.All about DSL,CABIL INTERNET,SATELLITE,FIBER OPTIC - Duration: 7:19.

hi friend tech samon channel ea apnader swagato,tech samon channel theke ami samrat.

For more infomation >> Different Betwin DIAL-UP VS BROADBAND.All about DSL,CABIL INTERNET,SATELLITE,FIBER OPTIC - Duration: 7:19.

-------------------------------------------

Un CHEVAL dans une salle de bain ???!!! / CSO #46 - Duration: 1:57.

For more infomation >> Un CHEVAL dans une salle de bain ???!!! / CSO #46 - Duration: 1:57.

-------------------------------------------

PREGUNTAS Y RESPUESTAS | Mateo Sánchez - Duration: 2:46.

For more infomation >> PREGUNTAS Y RESPUESTAS | Mateo Sánchez - Duration: 2:46.

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How to Earn Kohl's Cash

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Gyro Rotisserie recipe / Gyros aan 't spit recept - Duration: 4:16.

The gyros we are going to make, I make from a piece of Boston Butt.

First we take a bone out of it.

And some fat.

But of course it can also be done with a pork neck

On the other side there is still a little bit of fat, we are scrapping it off.

When this has happened, we will cut slices.

My jar of gyro herbs consists of:

paprika, oregano, white pepper, black pepper and salt.

I got this jar of gyro herbs from Vasiliki.

Vasiliki, she's from Greece, so she will know how to make gyro herbs.

When we have done one side, we turn the meat

and seasoning the other side also.

We also rub this side.

Then we do everything in an alu tray.

We place this covered in the frigde

and tomorrow we will continue with it .

The next day I'm going to spit the pieces of pork on the spit.

Fold the meat in half.

And so you easily string them on the skewer.

I cut off some loose pieces of meat.

Then place the clamps and my gyros skewer is ready.

Then we place it in the holder.

And we add a few nice pieces of wood.

I will be cooking the gyro skewer, to a core of about 70°C. / 158°F.

And when we have reached it, my gyro skewer is ready.

We get the meat from the skewer.

And cut the meat into strips.

I must say it smells really fantastic.

Oven baked garlic, coriander naans

my gyro meat

and of course tzatziki sauce.

I would say enjoy.

This is good!

Awesome!

Until next time

BensBQ.

For more infomation >> Gyro Rotisserie recipe / Gyros aan 't spit recept - Duration: 4:16.

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Opel Mokka X 1.4T 140pk Innovation AUTOMAAT - NAVI - - Duration: 0:56.

For more infomation >> Opel Mokka X 1.4T 140pk Innovation AUTOMAAT - NAVI - - Duration: 0:56.

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Pourquoi y a t-il des déserts sur Terre ? - Duration: 2:15.

For more infomation >> Pourquoi y a t-il des déserts sur Terre ? - Duration: 2:15.

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Try Not To Laugh Watching Funny Fail Compilation 2017 | Best Funny Videos of Life Awesome 2017 - Duration: 10:13.

Thanks for watching

Hope you have a great time

Please, like, comment and subscribe for more!!

For more infomation >> Try Not To Laugh Watching Funny Fail Compilation 2017 | Best Funny Videos of Life Awesome 2017 - Duration: 10:13.

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Lithophane-grad (from War Movie Still) - Duration: 2:30.

In this video I'm making a lithophane from a movie still.

The movie I have chosen to immortalise with this unusual technique is from the film Stalingrad,

but which one?

Well there are two version which you should watch.

They are like two sides of the same coin.

The first is the German version which plots the turning point in the second world war

from the perspective of the axis.

A pivotal scene comes about when troops receive an unexpected supply drop which included an

impalpable amount of food but more importantly a stash of medals,

sent with compliments of the Fuhrer.

At that point in the movie you are invited to join the disillusioned troops, and begin

to see through the propaganda machine in a way only the losing side can.

Medals as with statues and flags, don't win wars, they only embellish the grim realities of them.

The other is the Russian version.

This one's a little more upbeat.

It's most memorable scene is the one in which flaming soviets press forward through

a fuel explosion coming out the other side ablaze, and with their dying actions make

heavily entrenched fascists wet their pants.

I don't think you are meant to take this film as seriously as the first but in all

seriousness, I particularly like how it seamlessly blends absurdity with fighting fascism.

It is hard to decipher the desired effect of this movie.

Is it Putin porn, soviet realism or was the director a big fan of Quentin Tarantino?

In any case it received bad reviews, said to have tarnished the memory of the fallen

by giving the battle the 300 treatment but it also broke away from the traditional modus

operandi of the war movie, which is to make grown men feel a tiny shred of emotion, before

they push it further down into their masculinity sack.

For that reason alone the later is my preferred movie, and the scene I have chosen to make

this memento mori.

For more infomation >> Lithophane-grad (from War Movie Still) - Duration: 2:30.

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Motorcycle suspension 101 - Duration: 5:03.

I am Zé from Tea2Wine and welcome to Adventure Ride Chat the place were

all bikes are adventure and where the chat starts here with me and continues

with all of us down in that comment area, and if you are new to this segment of Tea2Wine

please do consider subscribing. There is a conversation that's very common

amongst riders, and that is "what is a good suspension". Well, first of all a

suspension, and suspension world, is mind-blowingly complicated and complex

for the large majority of people, and these new kinds bikes with their

electronic suspensions, well, they set the tone quite differently. But let's get

that part out and let's just talk about general rules, common rules, and mainly

mechanics suspensions. For that, what is a good suspension? For me, it's a suspension

that was designed for you, that was fine-tuned for you. And let's put this

into perspective. Let's say that you take Valentino Rossi bike and you take your

bike with the suspension fine-tuned for you, only your bike.

Ross's bike, his thing, his suspension his setup. Most likely, if you would do the

same route, you would say that his bike was not good and your bike was perfect

in terms of handling that is. And there is a good reason for it, because your

suspension was tuned for you, his, for his riding, for his feedback, for his way of

attacking turns and pushing in straights. It was designed for him.

So what can you do, what should you do? Let's first go from the beginning, and if you

get a bike, decide to take your bike to a suspension expert. Ride the bike a little

bit, get your feedbacks, take it to him, discuss it with him, and he will adjust

things from the air-gap, the kind of oil, the suspension rate... he is gonna adjust a

lot of things that will make the bike better for you. With this, is it important for

you to buy a top grade suspension and fine-tune it? Well, if you can afford it

Yes. But is it necessary? I would say that for the average rider no.

The suspension that comes with your bike, and if you fine tune it for

yourself, there is a strong chance that you're never gonna use all the potential

of that suspension, and if you do, well, that's great, because you're gonna feel

the improvement towards a better upgraded suspension, that again you're

going to fine tune for you. So take it as steps instead of just jumping to a very

very good thing, fine tuning it for you - and if you can afford it go for it - but

for the average rider, this is gonna be a jump that means they're gonna have a

product that they will not use fully to their extent. So, save some money if you

can and just buy what you need. Fine-tune it for you and take the best out of it.

There is something however that you can do without taking it to an expert, all

you need is a friend, a couple of wrenches and a notebook, and you can

adjust the sag of your suspension. Really really early day bikes will not have the

fine tunes needed for that, but the large majority of bikes from the eighties

forward will have at least certain things that you can do with preload.

Sot that will allow you to adjust the sag. I'm not gonna explain because I do

not have a bike here and it would be difficult just with my little usual

pop-ups to explain that to you, but I am gonna pop here this video from the MC

garage guys, and they explain perfectly all you need to do to adjust your sag,

and let me tell you, that's gonna allow you to ride a lot better.

But what is sag? Sag just mainly means what the suspension will travel once you

are on it, and what's left or not from the suspension travel

so, everything that the suspension can do. Imagine that your suspension is 30

centimeters when you're not sitting on top of it, and you sit on

top of it - let's imagine you're heavy - and the suspension only has five cm to move, so

that can be fine-tuned, that can be adjusted without the help of an expert.

With all of this in mind, what I want you to retain is that a good suspension for

person A is not necessarily a good suspension for person B. Do not buy into

"this is the best suspension in the world". It may be after it's fine-tuned for you.

As long as you're riding something that was not fine-tuned for you, it's

pointless if it is the most expensive suspension on earth or not. It's

pointless if it is the best suspension on this guy's or that guy's hands. It

needs to be tuned for you. With this piece of advice I say goodbye to 2017, I

say goodbye to all of you guys, and of course next time it's gonna be already

2018, the brand new year and I'm gonna be here to welcome you with fresh new

content. Until then, I wish you a great New Year!

For more infomation >> Motorcycle suspension 101 - Duration: 5:03.

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Disneyland, New Year's Eve, and Y2K - Duration: 3:48.

In just a few days we'll all be ringing in the new year and with that comes all of

the typical traditions surrounding it.

Noise makers.

Fireworks.

Wearing adult diapers while you're trapped in Times Square in freezing weather.

Yeah. Look it up. People actually do that.

The Disney parks are a popular vacation spot to welcome the new year, making it one of

the most crowded seasons to visit.

They're usually prepared for the crowds, but did you know one year they were also prepared

for disaster?

The New Years switch-over from 1999 to 2000 was a big one.

Besides being the year everyone was celebrating the new millennium it was one filled with

uncertainty all because of Y2K.

Y2K, for those too young to remember, was a computer bug that was anticipated years

before 2000 actually arrived.

The fear was that software that represented the year with the last two digits would switch

over from 99 to 00, and the computer programs wouldn't be able to tell the difference

between the year 1900 and the year 2000.

This would, in theory, cause all sorts of problems with all sorts of computer systems.

The issue wasn't worth ignoring, but with years to update software as needed to avoid

the bug, the problem wasn't much of an actual problem.

However that didn't prevent months of ever increasing fear over what potential apocalyptic

catastrophe we would meet when the clock struck 12 that New Years Eve.

Some people were taking survival courses, stockpiling supplies, and even preparing bunkers.

They genuinely thought this Y2K bug might trigger the downfall of civilization.

The world wasn't going to end on January 1st, 2000, but Disney wasn't going to chance it.

For their own Y2K prep, Disney planned to shut down select attractions in Disney between

11:45pm and 12:15am that would be especially troublesome to evacuate in the event something

did happen.

The list included Peter Pan's Flight which had guests suspended in hanging ride vehicles

and both Rocket Rods in Tomorrowland and the Haunted Mansion in New Orleans Square which

both required elevators to access.

According to an LA Times article the planning went beyond just shutting down attractions

for a while.

All of the departments at the park had access to cell phones in the event that landlines

malfunctioned at midnight.

In the event those cell phones stopped working, they also were outfitted with walkie talkies.

And if the walkie talkies failed?

Well, each department had designated runners who would be responsible for hand delivering

messages during a coordinated evacuation.

All departments were also provided with Y2K labeled flashlights in the event of a wide

scale power outage.

Now looking back, those precautions might have seemed like overkill, but considering

the competition, it wasn't so bad.

Over in California for instance, Universal Studios and Knott's Berry Farm decided to

play it safe and close rides for the night at 11:45pm.

Magic Mountain closed its doors entirely at 8pm that night.

Obviously when January 1st, 2000 arrived, nothing really happened.

There were a few isolated reports of software issues, but nothing that wasn't eventually

fixed.

Planes weren't falling from the sky, power grids weren't going offline, and thankfully,

the only thing going on at the Disney Parks was celebration.

For more infomation >> Disneyland, New Year's Eve, and Y2K - Duration: 3:48.

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How to Make Amazing Pinball Game Machine from Cardboard - Duration: 5:12.

Thanks for watching

Hope you have a great time

Please, like, comment and subscribe for more!!

For more infomation >> How to Make Amazing Pinball Game Machine from Cardboard - Duration: 5:12.

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Cringe-Worthy Questions Reporters Asked During Interviews - Duration: 4:39.

There are a ton of mundane celebrity interviews out there, but every now and then you get

one that really stands out.

In the following cases, what really separates the wheat from the chaff are the bizarre questions

reporters felt the need to pose, and the equally awkward (and sometimes awesome) reactions

those questions garnered.

Check out which ill-conceived interview questions had us cringing in our seats.

Types of trees

When Barbara Walters interviewed screen legend Katharine Hepburn in 1981, the actress compared

her own strong-willed personality to that of a tree for its sturdiness.

"I'm a very strong -- I've become a sort of… you know, tree."

"You know a tree or something."

And instead of letting that odd analogy go, Walters pressed on with it and asked:

"What kind of a tree are you?

If you think you're a tree?"

"I hope I'm not an elm."

Walters has never really lived down the bizarre question, although she's certainly got some

other highlights for her blooper reel:

"Is it all you?"

"Well, I can't show you."

"Why didn't you have your nose fixed?

Everybody had to say you should have your nose fixed?

How did you know it was going to be right?"

Literacy and exhaustion

Model-turned-actress Cara Delevingne was at the receiving end of a particularly awkward

line of questioning when, in July 2015, she appeared on a local Sacramento morning show

to promote her film Paper Towns.

Things started out on a sour note when she was asked whether she'd ever read John Green's

book upon which the film was based.

"The book is taught at a lot of high schools.

Did you get a chance to read it or do you even have time to read?"

Delevingne was obviously annoyed or offended by the question because she snapped back:

"No, I never read the book or the script actually, I kind of winged it."

Things continued to disintegrate with the interview, to the point that one of the anchors

made the comment:

"I saw you in London talking a couple of weeks ago on TV and you seemed a lot more excited

about it than you do right now.

Are you just exhausted?"

Delevingne was visibly taken aback by the question and insisted she was still jazzed

about the movie, but that didn't end the awkwardness.

"You do seem a bit irritated.

Perhaps it's just us."

"Yeah, I think it's just you."

Super Bowl switcheroo

Although there are a lot of Hollywood doppelgangers who don't mind getting mistaken for one another,

Samuel L. Jackson will not tolerate being mistaken for Laurence Fishburne.

A Los Angeles news reporter made the grave mistake of asking Jackson:

"That Super Bowl commercial, did you get a lot of reaction to thatSuper Bowl commercial?"

The reporter was obviously referring to the year's buzzy Matrix-themed car commercial,

but Jackson didn't star in that one.

"What Super Bowl commercial?"

Once the reporter realized his mistake, Jackson lashed back anyway, saying:

"See, what, you're as crazy as the people on Twitter."

The reporter owned up to the mistake and apologized for the error, but Jackson wasn't done dunking

him just yet.

"We don't all look alike!

We may be all black and famous but we don't all look alike."

"You're the entertainment reporter for this station and you don't know the difference

between me and Laurence Fishburne!?"

Jackson went on to list all of the commercials that he and other black actors had done to

both rub the anchor's nose in his mistake and better prepare him for next time.

Fleeting moment

Heiress and reality show veteran Paris Hilton was on the receiving end of an offensive question

during her 2011 appearance on Good Morning America when the correspondent asked her:

"Do you ever worry about your moment having passed?"

Hilton had already been eyeing the exit sign and decided to walk off camera after basically

being asked if her 15 minutes were over.

To her credit, though, she did return with a well-rehearsed rebuke:

"I've been in this business for 15 years now, so it's been a very long time, and just like

any other businessperson or someone in the industry, it's always important to reinvent

yourself and come up with new projects."

Shutting it down

British actor Tom Hardy has played a number of tougher-than-nails characters on screen.

And he definitely wasn't taking any guff in 2015 when a reporter at the Toronto Film Festival

discussed how is character in Legend contrasted with his own public persona.

"In the film your character Ronnie is very open about his sexuality, but given interviews

you've done in the past your own sexuality seems a bit more ambiguous."

Hardy's face clearly indicated that this wasn't a desirable direction for the question, but

the reporter then ventured to ask him:

"Do you find it hard for celebrities to talk to media about their sexuality?"

This time, it was director Brian Helgeland's face that said it all, and Hardy was not one

bit amused.

"What on earth are you on about?"

Hardy then asked the reporter to repeat his question, and things got even more awkward.

"I don't find it difficult for celebrities to talk about their sexuality… are you asking

me about my sexuality?"

"Um, sure."

"Why?"

You could cut the tension with a knife.

Thanks for watching!

Click the Nicki Swift icon to subscribe to our YouTube channel.

Plus check out all this cool stuff we know you'll love, too!

For more infomation >> Cringe-Worthy Questions Reporters Asked During Interviews - Duration: 4:39.

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The Kate by Tiny House Building Company | Gorgeous Small House Design - Duration: 2:06.

The Kate by Tiny House Building Company

For more infomation >> The Kate by Tiny House Building Company | Gorgeous Small House Design - Duration: 2:06.

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Is salt REALLY as unhealthy as you've been led to believe? - Duration: 10:31.

Hey everybody out there.

Coach Dan Long with Get Lean in 12.

And I have some awesome things I want

to go over about salt today.

So if you're curious about salt and what's good for you

and what's bad for you, you got to listen up.

Now, you're going to see a link up here.

Don't tap that link just yet.

I'm going to have something for you at the end of this video.

But first I want to talk about salt.

What salt is the best salt?

Now today, I have 2 different salts

here that I just wanted to show you and compare.

This is a sea salt and this is a Himalayan salt.

I know, it matches my shirt.

Did I do that on purpose?

Probably.

So the Himalayan salt is awesome for you.

Sea salt is awesome for you.

But what about table salt?

So minerals are absolutely vital in our diet.

They have to be in our diet, otherwise,

our body is not working at optimal level and pace.

Correct?

Our bodies can't produce all the minerals

that we need in function to be able to function properly.

So, how do we get those minerals into our body?

We have to eat them.

And so by eating minerals, like salt--

the correct salt-- it actually gives you a ton of benefits.

So let me give you some of those benefits now.

Salt regulates water content in the body and brain and blood

pressure.

Obviously, too much can cause higher blood pressure,

so we have to monitor that.

In particular, sodium chloride and potassium chloride

is a benefit that our body needs.

It also promotes healthy blood sugar levels.

So if you're not getting enough salt in your diet,

it's important to make sure that you're

incorporating these minerals because it

regulates blood sugar levels.

Also, various salts are important for bone

strengthening including calcium.

So if you want strong bones, you've

got to make sure you're getting your salt content.

It's also necessary for muscle contraction, in particular,

calcium.

That's how the muscles contract.

It's very important that you get that.

Promotes nutrient absorption in the intestine.

Many salt ions are cofactors that activate enzymes.

These include magnesium, manganese, iron, cobalt,

nickel, copper, and zinc.

All essential minerals that we need for proper body function.

Salts promote transport of substrates,

like sodium, potassium, calcium, and magnesium,

which is important for the function

of the cells in the body.

Important-- the minerals in our diet

are essential for a variety of bodily functions.

So, if you don't have any of this kind of salt--

that today that's why I wanted to bring this up to you.

This is important that you incorporate

some of these salts in your diet and not just

table salt, which I'm going to talk about here in a second.

Table salt-- man, I can't shake my head

enough about table salt. Trust me,

when I tell you what we have going on the table salt

these days, you're not going to want to ever consume that ever

again.

1 of the best ways to obtain these vital minerals

into your diet is to eat the salt. Now,

you've got to eat the correct salt,

and that's why I'm introducing you to these salts today.

Now, refined salt, you can find in the grocery store.

It's pretty common.

It's all over inside grocery stores.

Morton's one of the biggest sellers of this type of salt.

Salt in its natural form is referred to as unrefined salt.

So unrefined salt is a Himalayan salt,

which is coming from a rock.

And also, sea salt, which comes from what?

The sea.

Correct.

All right.

So unrefined salt has not been altered by humans,

so remember that.

Unrefined salt-- not been altered by humans.

This kind of salt contains many different minerals and elements

that are essential for our bodies.

For example, unrefined salt, such as Himalayan salt,

which I'm going to go ahead and show you some of this.

So I'm going to pour some of this in here so you can see.

It's such a beautiful salt. Now I'm

going to pour a little bit in my hand here, so you can see.

See how coarse it is, and see how the color is like a pink.

It's like a pink.

It's Himalayan salt. It's beautiful.

It tastes amazing, and it has a ton of essential minerals

that you need and I need for our diet.

So now, Himalayan salt is over 250 million years old

that has been exposed to high tectonic pressure surrounded

by ice and snow high in the mountains.

That's what Himalayan salt is, i.e.

why the name Himalayan.

When observed under a microscope,

this salt has a perfect crystalline structure,

which you can see.

Again, 1 more time.

Let me just show you here.

All right.

I hope you can see that.

Yeah, see that.

It's an amazing structure.

This salt is incredible for you.

It is believed to be the highest grade salt available.

Now, sea salt is also unrefined, and it's also amazing too.

But Himalayan salt-- that's why I want to bring this up--

you should be consuming 1 of the 2

of these kind of salts and not table salt.

And we're going to go over that in a second.

This salt has an unlimited shelf life,

and doesn't require any pouring agents.

That's why it's better than table salt.

Now, this is contrasted with refined salt. Now,

refined salt, remember what I said,

that's been touched by humans from the color to the shape

to the texture.

It contains 97.5% sodium.

This is refined salt with the rest being added chemicals that

are inside your salts, all of which,

you do not want in your food.

That's why you have to consume some good salts instead

of those salts, otherwise, it actually

can kill us faster than an amazing Himalayan salt.

Now, why is salt refined anyway?

Why do we even do that to such an amazing salt?

Why would we even waste our time?

Now sea salt, let me show you this by the way.

Let me put some of this in here.

Again, this is coarse and you can see it's also coarse,

and it also has a texture.

This is sea salt. Sea salt is also another amazing salt.

But, why do we refine salt anyways?

1 reason, for shelf life, just like pasteurized foods and that

stay on certain shelf lives forever.

And you also have foods that sit out there,

and they're processed.

And they sit there forever.

It's the same thing when it goes for salt. Salt, when it's

refined is done for shelf life.

Refined salt having all of its minerals removed.

It's purified.

It ensures a long shelf life.

In fact, refined salt in the grocery store

will stay on the shelf forever to maximize profits.

That's the only reason why salt is refined.

Manufacturers believe that all white salt product will

look cleaner to the consumer, which is us,

and, therefore, increases their sales,

which gives them more money.

Now, I don't know about you, I don't

want to give them more money so I can die faster.

So refined salt, it's bleached.

And that's why it's white.

It's bleached, in order to obtain that color.

Iodine is added to refine the salt

to prevent the swelling of the thyroid.

But there is insufficient iodine in refined salt

to prevent thyroid illnesses or to provide

for the body's iodine needs.

So now, if you have a choice, what are you going to choose?

I don't even have refined salt here

because I don't carry that in this household.

My wife and I are against it totally.

And I hope you are too because we

don't want you spending money on something

that's not good for you.

Sea salt is great.

So if you have a choice, it's simple.

After what I just said, it's simple to me.

You have Himalayan salt and sea salt. Himalayan being they say,

the best salt on the planet, in the world.

And then, sea salt, also another great salt that

provides up to 80 minerals--

up to 80 minerals.

Refined salt-- pretty much 0.

So cut clear.

Very cut clear here.

Himalayan salt. Sea salt. You know

which 1 that you should get.

Now, just for being here, I got a link up here.

Now part of your diet is part of exercise.

You need to be dieting and exercise 24/7.

And you should be exercising at least 12 minutes per day.

And that's why I have something specific for you

for being here today.

Because I know all the people from around the world,

like yourself, that are watching that

get this great information, you need

a 12-minute bursting exercise program

to go with your diet and your salt

because you want to live long.

And if you want to live long, I have the specific thing

you need right up here at this link.

You can click that link.

When you click that link, you're going

to go to product called the Over 40 Ab Solution.

Now, my buddy Sean and I and our wives

use this specific protocol.

It's only 12 minutes to get the bodies that we desire,

and we're all over the age of 40.

As a matter of fact, Sean's wife is 57.

My wife, she's turning 41.

And I'm actually 44, and Sean's 47.

Now we all stay fit and, we all help tons of people

around the world like yourself.

And we wanted to give you this link

up here that you can click now.

It will take you to the Over 40 Ab Solution

that you can have a proper diet and add

in just 12 minutes of exercise 3 to 5 times a week.

And guess what will happen?

You will get the body that you desire.

Now, tell me who you are.

Say hi.

Put some comments down below.

If you want like, give me a heart, or share.

I would love to see you on the next video.

And I want to thank you again for being here with me today.

I am Dan Long with Get Lean in 12.

And keep going strong.

For more infomation >> Is salt REALLY as unhealthy as you've been led to believe? - Duration: 10:31.

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Post Malone - I Fall Apart (Perto & Made By Tsuki Remix) [Lyrics] - Duration: 3:05.

♪ She told me that I'm not enough ♪

♪ And she left me with a broken heart ♪

♪ She fooled me twice and it's all my fault ♪

♪ She cut too deep, now she left me scarred ♪

♪ Now there's too many thoughts goin' through my brain ♪

♪ And now I'm takin' these shots like it's novacane ♪

♪ (Yeah) ♪

♪ Oooh, I fall apart ♪

♪ Down to my core ♪

♪ Oooh, I fall apart ♪

♪ Down to my core ♪

♪ (Yeah) ♪

♪ Oooh, I fall apart ♪

♪ Down to my core ♪

♪ Oooh, didn't know it before ♪

♪ Surprised when you caught me off guard ♪

♪ Hard as damn jewelry I bought ♪

♪ You was my shorty, I thought ♪

♪ Everybody told me so ♪

♪ Feelin' like I sold my soul ♪

♪ Devil in the form of a whore ♪

♪ Devil in the form of a whore ♪

♪ You said it ♪

♪ No you said it ♪

♪ No you said it ♪

♪ We'd be together ♪

♪ (Oooh) ♪

♪ (Yeah) ♪

♪ Ice keep droppin' and the drink keep flowin' ♪

♪ Try to brush it off but it keep on goin' ♪

♪ All these scars, can't help from showin' ♪

♪ Whippin' in the foreign and the tears keep blowin', yeah ♪

♪ Oooh, I fall apart ♪

♪ Down to my core ♪

♪ Oooh, I fall apart ♪

♪ Down to my core ♪

♪ Oooh, didn't know it before ♪

♪ Surprised when you caught me off guard ♪

♪ Hard as damn jewelry I bought ♪

♪ You was my shorty, I thought ♪

For more infomation >> Post Malone - I Fall Apart (Perto & Made By Tsuki Remix) [Lyrics] - Duration: 3:05.

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НОВОГОДНИЙ РЕЙД | Cam Pranks — Пранки c камерами - Duration: 8:50.

For more infomation >> НОВОГОДНИЙ РЕЙД | Cam Pranks — Пранки c камерами - Duration: 8:50.

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Hah Hah! POTUS Trump Trolls Global Warming Zealots as Record Fre - Duration: 2:05.

Hah-Hah!

POTUS Trump Trolls Global Warming Zealots as Record Freezing Temps Grip US

The United States is gripped in a freeze as record chill temps ravage parts of the country.

President Trump trolled global warming zealots Thursday on Twitter and celebrated the fact

that we aren�t paying trillions of dollars to fight the hoax.

Please president Trump, keep tweeting!

Trump tweeted Thursday, �In the East, it could be the COLDEST New Year�s Eve on record.

Perhaps we could use a little bit of that good old Global Warming that our Country,

but not other countries, was going to pay TRILLIONS OF DOLLARS to protect against.

Bundle up!�

President Trump has accurately called Climate Change a hoax.

Climate Change is a global redistribution of wealth based on junk science.

The U.S. should NEVER have to answer to foreign bureaucrats; we are a sovereign nation.

Trump kept a campaign promise when he pulled out of the Paris Climate Accord which would

have robbed Americans of wealth and freedom.

The US is getting blasted with record freezing temps.

Forecasters are saying we may see record freezing temps in the Northeast for New Years Eve.

We sure could use some of that global warming!

News of the freeze dominated headlines on the Drudge Report.

For more infomation >> Hah Hah! POTUS Trump Trolls Global Warming Zealots as Record Fre - Duration: 2:05.

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Rhonda Shear "Ahh Dreams" Hooded Poncho - Duration: 6:28.

For more infomation >> Rhonda Shear "Ahh Dreams" Hooded Poncho - Duration: 6:28.

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DNA sequencing by Maxam Gilbert method - Duration: 18:02.

Hello friends today in this video I am going to discuss about the DNA sequencing by Maxim Gilbert method

Okay, so at first

What is DNA sequencing DNA sequencing is a process which is used to know

the sequence or order of nucleotides

present in a DNA molecule, okay, so now this DNA sequencing technique was discovered by

maxam and Gilbert

And that's why this DNA sequencing technique is called the maxam

Gilbert DNA sequencing

Which is also known as the chemical degradation method because this DNA sequencing technique is

based on the chemical degradation

that means some chemical reagents are required to cut or

degrade the DNA molecule at a specific nucleotide

And that is the most important for this DNA sequencing and thats why this is called the chemical degradation

Method okay, so now let's talk about the whole DNA sequencing method step by step

Okay, so the first step is end level

End leveling means the labeling of DNA molecule by radioactive

Isotope at the both End of the DNA molecule

Okay, so now

Suppose. This is a DNA molecule

This is the DNA molecule which we want to sequence okay

And we all know that at the 5' position the phosphate group is present which is normal phosphate not

Radioactively labeled phosphate, okay, so now we have to replace this normal phosphate group at the 5 prime position

with the

Radioactive isotope of phosphate that is P 32 okay

So for that reason at first we have to remove this

Normal phosphate by the alkaline phosphatase which removed this

Normal phosphate, and then we get the dephosphorylated DNA molecule

so now we have to add the

radioactive phosphate at the 5 prime end ok and

Is done by polynucleotide kinase at the presence of ATP which

contains the radioactive P 32

Okay

So now this polynucleotide kinase add the radioactive p 32 from the ATP

to the 5' end of the both strand of DNA

Okay, so now we get the end leveled DNA

Molecule okay, so now the problem is for this DNA sequencing we only require

One site only require the labeling at the one site not in both site

Okay

So for that reason we have to cut this DNA molecule

Into two fragments and take only one fragment for the further experiment, okay?

so for this cutting for this cut we have to

Treat with restriction enzyme so the next step is restriction enzyme digestion

So after treatment after treating with the restriction enzyme we get

the two fragment of DNA

molecule okay, so now in this case another problem is

if the if the one fragment is very much smaller than the other fragment then we have to

discard the smaller fragment and take the larger fragment for the further experiment, but if the if these

Fragments are more or less equal

Not not they're unequal but more or less same in size

then we have to take both the fragments and

sequence separately okay so for this experiment suppose we get a

Very small fragment and very large fragment, okay?

So we have to discard the small fragment and take the larger fragment for the further experiment, okay

So now we get the larger fragment and now the another problem in this DNA sequencing is

we get we

required only

The single stranded radiolabeled

DNA fragment, okay so for that reason

We have to generate this double-stranded DNA into single standard form so now the next step is

denaturation

so after denaturation we get the

Single-stranded form and we have to isolate those single standard form that contain the five prime

five prime phosphate that is

Radioactively-labeled okay because this is required for the detection of the DNA molecule in the

autoradiography, okay

So now we get the end lebeled single-stranded DNA and now we have to perform the chemical degradation

Which is the most important of this DNA sequencing okay now

I will discuss about the most important step of this DNA sequencing that is the chemical degradation, okay

so still now we get the labeled single-stranded DNA which we want to sequence and

Here suppose. This is the whole sequence of this single-stranded DNA

Now we have to take multiple copies of this single-stranded DNA

Into this four reaction mixture tubes that is the g-tube, a+g tube

C + T 2 tube and C tube and

each of these reaction mixture tubes contain some different chemical reagents which

Specifically cut the DNA at specific

nucleotide base, okay

So for example in g tube all the DNA will be cut at the guanine base and in a+g tube

all the DNA will be cut

in both adenine and guanine

base and in

C+T tube all the DNA will be cut at cytosine and thymine base and in C tube all the DNA will be cut at

The cytosine base, okay

but remember one thing that

The reaction mixtures are made in such a way that only one cut will occur per DNA that means

If there have multiple

Copies of the DNA of this DNA into suppose the A+ G tube so there are multiple

Adenine and guanine base are present and all this DNA suppose. This is a

This is G. This is a this is G ok so if a chemical reagent

randomly cut

Suppose if we randomly cut in this

adenine base

Then it will never cut in other any base

Because the only one cut will be occurred per DNA suppose in this DNA

molecule the

reagent randomly cut in this

Guanine base it will never cut in other base okay?

Now I will discuss about the whole mechanism of this cleavage action ok so at first in G

Tube as a reagent we use dimethyl sulfate which

Specifically modified the guanine base by adding methyl group at the guanine base so for example suppose

This is our DNA sequence and here the guanine is present so when we add diethyl sulfate

Then it will modify the guanine base, and then it will release that guanine base not the nucleotide okay

And then when we add piperidine which is another reagent, then it will

cut the phosphodiester bond

And then remove this nucleotide modified nucleotide ok so in this way cleavage will be occur okay

So now here in this g tube only the g base will be cut so

Here we can see that only 2 G is present okay

so we will get 2

fragments for this G and this G when this g will be removed then when it then we will get only the phosphate group and

When this g will be removed we will get this fragment. Okay, so in this G tube. We will get

Two fragments all together two fragments we will get

Okay, and in this tube that is a plus G tube. Also. We use

Dimethyl sulfate as the reagent but in presence of formic acid so in this condition

dimethyl sulfate

Specifically modified all the adenine base, but occasionally modified guanine base also

Okay, so in this case

We will

cut we will modify the adenine and also guanine base by the

Dimethyl sulfate and then piperidine will come and cut and remove that nucleotide. Okay, so here

We can see that all together one two three

four five six all together six

base are present and so we will get six fragment in this a plus g tube because

when this g will be removed we will get only the

This fragment and when a will be removed then we will get this fragment when this a will be removed

Then we will get this fragment and so on this fragment this G will be removed

We will get this fragment so in this way all together we get

six DNA fragments in this a plus G tube and in c+ t tube we will

We use

Hydrazine as a reagent and this hydrazine

specifically cut specifically break the

specifically break the ring structure of this cytosine and thymine base and in this way this hydrazine modify the

cytosine and thymine base and then piperidine will come and

recognize that

modified base, and then they cut and removes that

Nucleotide okay so in this way

the

DNA will be cut in this C plus T tube and here we can see that all together

one two three

Four five six bases six C and E bases are present in this DNA so there will be six

We will get six fragment

because when

This T will be removed

We will get this fragment when this t will be removed we will get this fragment and so on okay and this C tube

We use hydrazine also use hydrazine

but in 2Ml NaCl and this in this condition the

this condition the hydrazine will remove will modify only the

Cytosine based not the thymine base and then piperidine will come and cut and remove that cytosine base

base containing nucleotide, okay, so now so now here. We can see that all together

Two cytosine bases are present in that DNA so when this cytosine will be removed

We'll get this fragment when these cytosine will be remove we will get that fragment. Okay, so all together we will get

Two fragment and here we get six fragment

So this is the whole mechanism of this chemical degradation after chemical degradation is done now

We have to separate the DNA fragments which are present in those tubes

According to their size by high resolution gel electrophoresis, okay?

So and remember that the DNA fragments which are present in those tubes

will run in the different Lane, okay, and

the larger DNA fragment will migrate less in the gel and present on the top of the gel and the

lower or the smaller DNA fragment will migrate more in the

gel and present on the bottom of the gel okay, so now after gel electrophoresis is done

Then we have to see these bands by autoradiography, okay, so after autoradiography

Then we can see those bands

And then we have to determine the sequence or order of nucleotides which are present in this DNA

Okay, so now we know that our DNA

fragment is 12

Nucleotide long so the largest nucleotide long so the largest DNA fragment what we can get in this

Gel that is the 12 nucleotide long DNA fragment and that

12 nucleotide DNA fragment we get in the c tube in prominent and in high amount and in c-plus

T tube in low amount so from this we can say that

The last nucleotide of this 12 nucleotide DNA fragments will be C not

T because in c tube it will get we will get larger amount of

DNA and c+t tube we get only small amounts that will be that for the C, okay, so

as in the c tube the DNA will be cut in the C so we get

the bands in c tube which

Determined that the last nucleotide will be the 12th nucleotide will be the C

Okay, so the next is

11 nucleotides DNA fragments 11 nucleotides DNA fragment

We get in the C plus T tube, but not in the C tube, so it will be T

not c because we don't get any band in the c so the

11th number nucleotide will be the T. Okay will be the T and the

Next one is the same so this is also T

and the 9th

Nucleotide nine nucleotides DNA fragment we get in the A plus G tube

Okay, so as we don't get any bands in G tube

So we can conclude that there the last

last nucleotide of this nine nucleotides DNA fragment will be A not G because

They are in G Tube. No bands We get okay so the ninth

so the ninth nucleotide will be a

So the ninth nucleotide will be a and next

One is also same that is the a and the seven

Seven nucleotide the seven nucleotides DNA fragment we get in high among in G

But in low amount in A plus G so from this we can conclude that the seventh number nucleotide will be

G okay not a because here we get the bands

for g not a in low amount okay

So in this way, we can sequence all the nucleotides

and then we have to read this sequence from the bottom to top because the

First nucleotide is the G that is G and the last nucleotide 12 new is C

Okay,

this will be the

5 prime and this will be the 3 prime ok

So in this way, we can sequence we can determine the sequence of the DNA

Fragment and now the whole process is at first we have to label

we have to end leveling have to do end leveling that is the leveling the whole DNA in

both the end by radioactive isotope, then we have to cut the

labeled DNA in middle by restriction enzyme so that we can get the get the

radioactive isotope or labeled DNA at only one site

And then we have to do the double-stranded DNA into labeled single standard form and then we have to perform chemical

chemical degradation

in the

tubes reaction tubes where the

specific reagents will cut the DNA fragments will cut the DNA fragments at

Specific nucleotide base ok and then gel electrophoresis is done to separate the DNA

Different sized DNA fragments, and then we can see those bands by autoradiography

And then we have to determine the sequence of this

nucleotides ok so this is the whole process of Maxam and Gilbert DNA sequencing ok now there have some limitations or

disadvantages of this maxim Gilbert DNA sequencing the main two disadvantage of this

DNA sequencing the first one is in this DNA sequencing we use radioactivity and

Also be used toxic chemicals

And that was risky and the second disadvantage of this

DNA sequencing that is by this DNA sequencing we can sequence only up to

500 base pair okay, and that is the main disadvantage of this Maxam and Gilbert DNA sequencing, okay, thank you for watching this video

For more infomation >> DNA sequencing by Maxam Gilbert method - Duration: 18:02.

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Forbes on Fox 12/30/17 11AM | December 30, 2017 Breaking News - Duration: 34:01.

For more infomation >> Forbes on Fox 12/30/17 11AM | December 30, 2017 Breaking News - Duration: 34:01.

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Cavuto on Business 12/30/17 10AM | December 30, 2017 Breaking News - Duration: 34:26.

For more infomation >> Cavuto on Business 12/30/17 10AM | December 30, 2017 Breaking News - Duration: 34:26.

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Cavuto on Business 12/30/17 11AM | December 30, 2017 Breaking News - Duration: 40:37.

For more infomation >> Cavuto on Business 12/30/17 11AM | December 30, 2017 Breaking News - Duration: 40:37.

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Heather Fell's Cérvelo P2 | GTN's Presenter Pro Bike - Duration: 3:15.

- Well, this is my Cervelo P2, which I had the chance

to ride on a shoot in Mallorca.

I also got to race on it in Chattanooga

at the World Championships.

(relaxed electronic music)

I actually only got on a time-trial bike

for the first earlier this year, and rode another bike

for a couple of months.

I then got on this one,

and I was amazed at how smooth it felt and how easy to ride,

and it's also pretty slick and understated.

I genuinely really love this bike.

It's a 51-inch frame, and attached to that,

we've got profile-designed T2 Wing Aero base bars,

and then the Profile-designed T4 Plus Aero bars.

On the end of those, we've got the shifters

for the Shimano Ultegra DI2.

So I'm using Shimano Ultegra front and rear Derailleurs

then Shimano Dura-Ace chain ring at 52/36,

which was quite helpful for getting up the big climb

in Chattanooga and then an 11/28 cassette on the back.

As you can see, it's using the Quarq Power Metre,

and it's the very first time I've ever ridden with power,

so racing with it was quite a new experience

obviously I didn't need to look at the numbers

going up the hill but for the rest of it,

it was really interesting,

and I managed to stick to my plan pretty well.

I've got 172.5 length cranks on the end of those,

the Shimano Ultegra pedals.

As you can see, I put Shimano 105 rim brakes

on the front, and the back,

I'm using the Vision Metron wheels,

got the 55SL on the front, and the 81SL on the back,

and then on those, running the Continental Gran Prix tyres,

at 25 MM wide, and for Chattanooga,

I put in a pressure of 190

because the roads were a little bit rough in places.

For Cevelo Aero seat posts,

and then on top of the is the ISM Adamo Prologue

split-nosed saddle, and it's actually the first time

I've ever ridden a split nose saddle,

and compared to the time-trial bike

I was on previously, this is so comfortable,

I genuinely feel like I can ride this bike all day

with no discomfort.

As we're talking about comfort,

you can see that I've still got space

left in my position isn't that aggressive,

but being pretty new to time-travel bikes

this year, I very much wanted to be comfortable

and feel confident on the bike

and then be able to have that consistent power

that I needed to target.

Well as you can see since Chattanooga,

I have actually given the bike a bit of a clean,

removed all the extra bits,

but I stored my spares behind the saddle

for the race day, and then I actually use

profile-designed Aero bottoms for my Aero bars at the front,

and then I had a bottlecage on the downtube,

but there's also options on this bike

to have a storage box on the top

for any of you nutrition.

For you weight weenies out there,

it comes in at 8.9 KGs, and just remember though,

there are none of the extras on this at the moment,

but going through my bike for this pro bike

has made me realise how much I love the P2

and I think I should get back out on it,

I just need the weather to get a little bit nicer,

if you've enjoyed this, give it a thumbs up like,

and to subscribe to GTN for free,

just click on the globe,

and if you want to have a look at Mark's pro bike,

click up here, if you want to see me riding this bike

in action, then check out my Chattanooga blog down here.