Snickerstream is now back from the dead. You can stream your 3DS screen easily with this app
All you have to do is install custom firmware on your console then run BootNTR and you are ready to go.
This is my New 3DS XL.
And it is running custom firmware on SysNAND.
Now we need to install BootNTR. We will use FBI to install it. Make sure your console is connected to the internet.
Choose TitleDB. And then search for BootNTR Selector
Then install it
Now press any button and press B to return to the main menu then choose Remote Install
Choose Receive URLs over the network
This is your console's IP address and port number. Make a note of this.
Press B twice then press Start to exit FBI
Now run BootNTR Selector
Open the web browser and go to this URL
You can read what Snickerstream is from this page
Click the releases tab and download the latest version of Snickerstream
You can choose between 32bit or 64bit version.
Extract the package anywhere on your computer
Then run it
Enter the console's IP address then press Connect.
If done correctly, you will see your 3DS screen on your computer
You can press Up or Down to scale up or scale down the screen.
But you will start noticing some frame rate lags after scaling up the screen
Let's try playing a game
Too bad, you cannot use input redirection with this app for now.
This app is intended for streaming purpose only
Thank you for watching this short tutorial. I hope you liked it. Please subscribe for more upcoming videos
For more infomation >> 3DS Tutorial : How to stream 3DS Screen to Computer using Sneakerstream - Duration: 3:48.-------------------------------------------
НОВОГОДНИЙ РЕЙД | Cam Pranks — Пранки c камерами - Duration: 8:50.
For more infomation >> НОВОГОДНИЙ РЕЙД | Cam Pranks — Пранки c камерами - Duration: 8:50. -------------------------------------------
Different Betwin DIAL-UP VS BROADBAND.All about DSL,CABIL INTERNET,SATELLITE,FIBER OPTIC - Duration: 7:19.
hi friend tech samon channel ea apnader swagato,tech samon channel theke ami samrat.
-------------------------------------------
PREGUNTAS Y RESPUESTAS | Mateo Sánchez - Duration: 2:46.
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Un CHEVAL dans une salle de bain ???!!! / CSO #46 - Duration: 1:57.
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The future of shipping
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Capodanno in diretta da Maratea con Albano e Romina Power | K.N.B.T - Duration: 6:26.
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Uomini e Donne, Anna Tedesco lascia il programma? | M.C.G.S - Duration: 4:01.
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Uomini e Donne over: Gemma ed i suoi regali dall'Oriente per Giorgio Manetti | Wind Zuiden - Duration: 3:26.
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Romina power Al Bano Carrisi è molto deluso e arrabbiato | WInd Zuiden - Duration: 3:32.
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Flavio Briatore e le frasi infelici: "non so come si possa vivere con 1300 euro al mese." - Duration: 9:13.
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Renault Mégane Scénic 1.6E RN - Duration: 0:43.
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Quiet Time | Train Simulator 2018 | Stamford Trash | GE E-33 NH EF-4 | New York to New Haven - Duration: 1:04:30.
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Citroën Grand C4 Picasso 1.6 e-THP 7pl Intesive 1e eigenaar orig.nl geleverd RIJKLAARPRIJS - Duration: 1:01.
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Mercedes-Benz E-Klasse 320 AVANTGARDE A4, AUTOMAAT, SEDAN! - Duration: 1:00.
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Peugeot 108 1.0 e-VTi Blue Lease / 5-DRS / AIRCO / WEINIG KM - Duration: 0:57.
For more infomation >> Peugeot 108 1.0 e-VTi Blue Lease / 5-DRS / AIRCO / WEINIG KM - Duration: 0:57. -------------------------------------------
Mercedes-Benz E-Klasse Coupé 350 4Matic 306PK Prestige 7G-Tronic Plus AMG Pakket (51.023Km Sportled - Duration: 0:57.
For more infomation >> Mercedes-Benz E-Klasse Coupé 350 4Matic 306PK Prestige 7G-Tronic Plus AMG Pakket (51.023Km Sportled - Duration: 0:57. -------------------------------------------
Citroën C3 1.6 e-HDi Selection - Duration: 0:59.
For more infomation >> Citroën C3 1.6 e-HDi Selection - Duration: 0:59. -------------------------------------------
Citroën C1 1.0 e-VTi Feel 5-DRS. (Airco/Bluetooth) - Duration: 0:52.
For more infomation >> Citroën C1 1.0 e-VTi Feel 5-DRS. (Airco/Bluetooth) - Duration: 0:52. -------------------------------------------
Uomini e Donne news, Cristian e Tara si sono separati? Ecco la foto della verità - Duration: 4:22.
For more infomation >> Uomini e Donne news, Cristian e Tara si sono separati? Ecco la foto della verità - Duration: 4:22. -------------------------------------------
UeD, Cosa ha regalato Tina Cipollari a Gemma per questo Natale? | Wind Zuiden - Duration: 3:21.
For more infomation >> UeD, Cosa ha regalato Tina Cipollari a Gemma per questo Natale? | Wind Zuiden - Duration: 3:21. -------------------------------------------
Gossip, Cecilia Rodriguez: 'Ignazio Moser non mi sopporta più' | Wind Zuiden - Duration: 3:32.
For more infomation >> Gossip, Cecilia Rodriguez: 'Ignazio Moser non mi sopporta più' | Wind Zuiden - Duration: 3:32. -------------------------------------------
The Last Jedi psychology explained - Duration: 7:20.
Too many jokes!
Too many surprises!
Too many porgs!
There, now you don't need to write that in the comments section.
Let's move on to discussing how The Last Jedi is the most psychologically complex Star
Wars film ever made.
Star Wars has a lot to say about emotions.
The original trilogy encourages us to feel our emotions, quiet our minds, focus on the
present moment.
This is called mindfulness.
We see it when when Luke destroys the Death Star, during Yoda's Jedi training, and it's
what helps Darth Vader turn against the Emperor.
The prequel trilogy warns us about the dangers of avoiding emotions.
This is called experiential avoidance.
Anakin never wants to experience the pain of losing someone he loves ever again.
This is why he becomes obsessed with trying to protect Padme and it's what leads him
to the dark side.
The Force Awakens is mostly about new characters set against familiar Star Wars ideas.
It doesn't say anything new about emotions.
We see Rey use mindfulness and Kylo loses his temper, just like his grandfather and
a lot of people who struggle with experiential avoidance.
It does has some new ideas about heroism, but nothing about emotions.
The Last Jedi goes into a very different direction.
It's a story that explores the oldest, most universal emotion -- fear.
To explain how, I have to get into spoilers.
If you haven't watched The Last Jedi, pause this video, go see it because it's awesome,
and then come back here.
Star Wars has discussed fear before, but it did it badly.
In The Phantom Menace, Yoda says "Fear leads to anger, anger leads to hate, hate…leads
to suffering."
Fear doesn't necessarily lead to anger or suffering.
Fear is an emotion that is activated when we sense danger.
It transformers your body to run faster, jump higher, and fight harder.
It also focuses all of your attention on your immediate threat.
For a long time researchers have known fear leads to fight or flight - a drive to face
danger head on or run away to safety.
We see this a lot in The Last Jedi - Poe's instinct is to attack when threatened.
Finn is afraid of being captured by the First Order and tries to leave the resistance on
an escape pod.
Luke is afraid of who he has become and is hiding at the first Jedi temple on Ahch-To.
While fight and flight has dominated fear research, in 2000 psychologist Shelly Taylor
introduced a new idea: Tend and befriend.
Tending means taking care of yourself, those around you, and helping people to feel calm.
Befriending is about connecting with other people to make your community safer.
We see a lot of this in The Last Jedi too - Leia is focused on protecting the resistance
and connecting with allies.
Rey tries to help Luke and Kylo in the hopes that they will join the resistance.
Rose saves Finn's life and tells him, "We're going to win this war not by fighting what
we hate, but saving what we love."
We've never seen such a detailed exploration of fear before in Star Wars and we rarely
see it in Hollywood.
While everyone is capable of both, men tend to rely on fight or flight strategies while
women are more likely to tend and befriend.
Since most films are created by men, stories tend to glorify fight or flight while tend
and befriend is an secondary response at best and a cowardly act at worst.
The Last Jedi breaks this trend by showing us both approaches are equally important to
survival.
That's why the most awesome moment in this film belongs to Vice Admiral Holdo.
She is the integration of fight or flight and tend and befriend.
She spends the whole movie protecting the resistance but attacks when it's the only
way for the resistance to survive.
The Last Jedi also shows us what happens when we're haunted by our fears.
Luke sensed a powerful darkness growing in Ben Solo and for one brief moment activated
his lightsaber with the intention of killing Ben.
Ben destroyed Luke's Jedi temple, killed many of its students, and transformed into
Kylo Ren.
Luke is traumatized by this.
He runs away to Ahch-To, closes himself off to The Force, and decides the Jedi order must
die with him.
Luke's story is an honest representation of trauma.
It's not seeing something horrific that makes an event traumatizing.
Luke's seen plenty of people die before and he's killed a lot of people too.
Traumas happen when an event challenges your fundamental beliefs.
Luke was taught the Jedi are the guardians of peace and justice but now as a Jedi he's
contributed to his nephew's turn to the dark side and the rise of the First Order.
When we avoid talking about trauma, as Luke has, we get stuck.
The normal healing that's supposed to happen never does.
Luke is stuck thinking about his guilt letting down Han and Leia, he's disgusted with Jedi
teachings, and ashamed about what he's become.
The only way to move forward after a trauma is by finding a way to integrate the experience
into your life.
This means talking about what happened, re-examining your role in the event, and revising your
beliefs to accurately reflect the trauma.
This is what Rey and Yoda help Luke to do, realize that he can learn from his failure
and help those who are in danger.
That's why the second most awesome moment in this film is seeing Luke confront his trauma
through a peaceful nonviolent sacrifice.
The Last Jedi defies our expectations and challenges our fundamental beliefs about Star
Wars, heroes, and villains.
I know a lot of fans are struggling with what happens to Luke Skywalker.
The original trilogy is a mythical heroes journey about Luke's call to adventure,
transformation into a Jedi, and restoring peace to the galaxy.
That's how myths work: heroes always do good and they defeat evil.
But myths aren't real.
Sometimes heroes make mistakes and have to live with the consequences.
The Last Jedi is an honest account of what happens to real life heroes, what it means
to be brave in the face of fear, and the consequences of facing traumatic situations.
Like Luke, Star Wars was stuck in myth.
But you can't renew Star Wars by repeating mythical formulas.
By embracing reality, Star Wars has grown beyond what it was and is reborn into something
completely new.
What did you think of The Last Jedi?
Let me know in the comments below.
There's one more way we can react to fear - we can freeze.
We see that when Kylo Ren intends to kill his mom but freezes when the moment comes.
I didn't talk about that earlier because this video is already longer than what I intended
and I couldn't find a way to easily squeeze it in so I'm putting it here.
-------------------------------------------
Ogni vita dovrebbe essere miracolosa (Everyone's Life Should Be Miraculous) - Duration: 28:19.
For more infomation >> Ogni vita dovrebbe essere miracolosa (Everyone's Life Should Be Miraculous) - Duration: 28:19. -------------------------------------------
Seat Leon 2.0 TDI Businessline Automaat Navi Elekt Pakket 159.000 KM - Duration: 1:27.
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王大治升级当爸爸，网友：他和董洁什么时候结束的？ - Duration: 11:49.
For more infomation >> 王大治升级当爸爸，网友：他和董洁什么时候结束的？ - Duration: 11:49. -------------------------------------------
Family fun on the water For more infomation >> Family fun on the water-------------------------------------------
Renault Captur 90pk TCe Dynamique | Camera | Climate Controle | R-LINK | Cruise Controle | Metallic - Duration: 0:54.
For more infomation >> Renault Captur 90pk TCe Dynamique | Camera | Climate Controle | R-LINK | Cruise Controle | Metallic - Duration: 0:54. -------------------------------------------
Volvo V40 T2 122PK R-Design - Duration: 0:59.
For more infomation >> Volvo V40 T2 122PK R-Design - Duration: 0:59. -------------------------------------------
3DS Tutorial : How to stream 3DS Screen to Computer using Sneakerstream - Duration: 3:48.
Snickerstream is now back from the dead. You can stream your 3DS screen easily with this app
All you have to do is install custom firmware on your console then run BootNTR and you are ready to go.
This is my New 3DS XL.
And it is running custom firmware on SysNAND.
Now we need to install BootNTR. We will use FBI to install it. Make sure your console is connected to the internet.
Choose TitleDB. And then search for BootNTR Selector
Then install it
Now press any button and press B to return to the main menu then choose Remote Install
Choose Receive URLs over the network
This is your console's IP address and port number. Make a note of this.
Press B twice then press Start to exit FBI
Now run BootNTR Selector
Open the web browser and go to this URL
You can read what Snickerstream is from this page
Click the releases tab and download the latest version of Snickerstream
You can choose between 32bit or 64bit version.
Extract the package anywhere on your computer
Then run it
Enter the console's IP address then press Connect.
If done correctly, you will see your 3DS screen on your computer
You can press Up or Down to scale up or scale down the screen.
But you will start noticing some frame rate lags after scaling up the screen
Let's try playing a game
Too bad, you cannot use input redirection with this app for now.
This app is intended for streaming purpose only
Thank you for watching this short tutorial. I hope you liked it. Please subscribe for more upcoming videos
-------------------------------------------
НОВОГОДНИЙ РЕЙД | Cam Pranks — Пранки c камерами - Duration: 8:50. For more infomation >> НОВОГОДНИЙ РЕЙД | Cam Pranks — Пранки c камерами - Duration: 8:50. -------------------------------------------
Different Betwin DIAL-UP VS BROADBAND.All about DSL,CABIL INTERNET,SATELLITE,FIBER OPTIC - Duration: 7:19.hi friend tech samon channel ea apnader swagato,tech samon channel theke ami samrat.
-------------------------------------------
Un CHEVAL dans une salle de bain ???!!! / CSO #46 - Duration: 1:57. For more infomation >> Un CHEVAL dans une salle de bain ???!!! / CSO #46 - Duration: 1:57. -------------------------------------------
PREGUNTAS Y RESPUESTAS | Mateo Sánchez - Duration: 2:46. For more infomation >> PREGUNTAS Y RESPUESTAS | Mateo Sánchez - Duration: 2:46. -------------------------------------------
How to Earn Kohl's Cash For more infomation >> How to Earn Kohl's Cash-------------------------------------------
Gyro Rotisserie recipe / Gyros aan 't spit recept - Duration: 4:16.
The gyros we are going to make, I make from a piece of Boston Butt.
First we take a bone out of it.
And some fat.
But of course it can also be done with a pork neck
On the other side there is still a little bit of fat, we are scrapping it off.
When this has happened, we will cut slices.
My jar of gyro herbs consists of:
paprika, oregano, white pepper, black pepper and salt.
I got this jar of gyro herbs from Vasiliki.
Vasiliki, she's from Greece, so she will know how to make gyro herbs.
When we have done one side, we turn the meat
and seasoning the other side also.
We also rub this side.
Then we do everything in an alu tray.
We place this covered in the frigde
and tomorrow we will continue with it .
The next day I'm going to spit the pieces of pork on the spit.
Fold the meat in half.
And so you easily string them on the skewer.
I cut off some loose pieces of meat.
Then place the clamps and my gyros skewer is ready.
Then we place it in the holder.
And we add a few nice pieces of wood.
I will be cooking the gyro skewer, to a core of about 70°C. / 158°F.
And when we have reached it, my gyro skewer is ready.
We get the meat from the skewer.
And cut the meat into strips.
I must say it smells really fantastic.
Oven baked garlic, coriander naans
my gyro meat
and of course tzatziki sauce.
I would say enjoy.
This is good!
Awesome!
Until next time
BensBQ.
-------------------------------------------
Opel Mokka X 1.4T 140pk Innovation AUTOMAAT - NAVI - - Duration: 0:56.
For more infomation >> Opel Mokka X 1.4T 140pk Innovation AUTOMAAT - NAVI - - Duration: 0:56. -------------------------------------------
Pourquoi y a t-il des déserts sur Terre ? - Duration: 2:15.
For more infomation >> Pourquoi y a t-il des déserts sur Terre ? - Duration: 2:15. -------------------------------------------
Try Not To Laugh Watching Funny Fail Compilation 2017 | Best Funny Videos of Life Awesome 2017 - Duration: 10:13.
Thanks for watching
Hope you have a great time
Please, like, comment and subscribe for more!!
-------------------------------------------
Lithophane-grad (from War Movie Still) - Duration: 2:30.
In this video I'm making a lithophane from a movie still.
The movie I have chosen to immortalise with this unusual technique is from the film Stalingrad,
but which one?
Well there are two version which you should watch.
They are like two sides of the same coin.
The first is the German version which plots the turning point in the second world war
from the perspective of the axis.
A pivotal scene comes about when troops receive an unexpected supply drop which included an
impalpable amount of food but more importantly a stash of medals,
sent with compliments of the Fuhrer.
At that point in the movie you are invited to join the disillusioned troops, and begin
to see through the propaganda machine in a way only the losing side can.
Medals as with statues and flags, don't win wars, they only embellish the grim realities of them.
The other is the Russian version.
This one's a little more upbeat.
It's most memorable scene is the one in which flaming soviets press forward through
a fuel explosion coming out the other side ablaze, and with their dying actions make
heavily entrenched fascists wet their pants.
I don't think you are meant to take this film as seriously as the first but in all
seriousness, I particularly like how it seamlessly blends absurdity with fighting fascism.
It is hard to decipher the desired effect of this movie.
Is it Putin porn, soviet realism or was the director a big fan of Quentin Tarantino?
In any case it received bad reviews, said to have tarnished the memory of the fallen
by giving the battle the 300 treatment but it also broke away from the traditional modus
operandi of the war movie, which is to make grown men feel a tiny shred of emotion, before
they push it further down into their masculinity sack.
For that reason alone the later is my preferred movie, and the scene I have chosen to make
this memento mori.
-------------------------------------------
Motorcycle suspension 101 - Duration: 5:03.
I am Zé from Tea2Wine and welcome to Adventure Ride Chat the place were
all bikes are adventure and where the chat starts here with me and continues
with all of us down in that comment area, and if you are new to this segment of Tea2Wine
please do consider subscribing. There is a conversation that's very common
amongst riders, and that is "what is a good suspension". Well, first of all a
suspension, and suspension world, is mind-blowingly complicated and complex
for the large majority of people, and these new kinds bikes with their
electronic suspensions, well, they set the tone quite differently. But let's get
that part out and let's just talk about general rules, common rules, and mainly
mechanics suspensions. For that, what is a good suspension? For me, it's a suspension
that was designed for you, that was fine-tuned for you. And let's put this
into perspective. Let's say that you take Valentino Rossi bike and you take your
bike with the suspension fine-tuned for you, only your bike.
Ross's bike, his thing, his suspension his setup. Most likely, if you would do the
same route, you would say that his bike was not good and your bike was perfect
in terms of handling that is. And there is a good reason for it, because your
suspension was tuned for you, his, for his riding, for his feedback, for his way of
attacking turns and pushing in straights. It was designed for him.
So what can you do, what should you do? Let's first go from the beginning, and if you
get a bike, decide to take your bike to a suspension expert. Ride the bike a little
bit, get your feedbacks, take it to him, discuss it with him, and he will adjust
things from the air-gap, the kind of oil, the suspension rate... he is gonna adjust a
lot of things that will make the bike better for you. With this, is it important for
you to buy a top grade suspension and fine-tune it? Well, if you can afford it
Yes. But is it necessary? I would say that for the average rider no.
The suspension that comes with your bike, and if you fine tune it for
yourself, there is a strong chance that you're never gonna use all the potential
of that suspension, and if you do, well, that's great, because you're gonna feel
the improvement towards a better upgraded suspension, that again you're
going to fine tune for you. So take it as steps instead of just jumping to a very
very good thing, fine tuning it for you - and if you can afford it go for it - but
for the average rider, this is gonna be a jump that means they're gonna have a
product that they will not use fully to their extent. So, save some money if you
can and just buy what you need. Fine-tune it for you and take the best out of it.
There is something however that you can do without taking it to an expert, all
you need is a friend, a couple of wrenches and a notebook, and you can
adjust the sag of your suspension. Really really early day bikes will not have the
fine tunes needed for that, but the large majority of bikes from the eighties
forward will have at least certain things that you can do with preload.
Sot that will allow you to adjust the sag. I'm not gonna explain because I do
not have a bike here and it would be difficult just with my little usual
pop-ups to explain that to you, but I am gonna pop here this video from the MC
garage guys, and they explain perfectly all you need to do to adjust your sag,
and let me tell you, that's gonna allow you to ride a lot better.
But what is sag? Sag just mainly means what the suspension will travel once you
are on it, and what's left or not from the suspension travel
so, everything that the suspension can do. Imagine that your suspension is 30
centimeters when you're not sitting on top of it, and you sit on
top of it - let's imagine you're heavy - and the suspension only has five cm to move, so
that can be fine-tuned, that can be adjusted without the help of an expert.
With all of this in mind, what I want you to retain is that a good suspension for
person A is not necessarily a good suspension for person B. Do not buy into
"this is the best suspension in the world". It may be after it's fine-tuned for you.
As long as you're riding something that was not fine-tuned for you, it's
pointless if it is the most expensive suspension on earth or not. It's
pointless if it is the best suspension on this guy's or that guy's hands. It
needs to be tuned for you. With this piece of advice I say goodbye to 2017, I
say goodbye to all of you guys, and of course next time it's gonna be already
2018, the brand new year and I'm gonna be here to welcome you with fresh new
content. Until then, I wish you a great New Year!
-------------------------------------------
Disneyland, New Year's Eve, and Y2K - Duration: 3:48.
In just a few days we'll all be ringing in the new year and with that comes all of
the typical traditions surrounding it.
Noise makers.
Fireworks.
Wearing adult diapers while you're trapped in Times Square in freezing weather.
Yeah. Look it up. People actually do that.
The Disney parks are a popular vacation spot to welcome the new year, making it one of
the most crowded seasons to visit.
They're usually prepared for the crowds, but did you know one year they were also prepared
for disaster?
The New Years switch-over from 1999 to 2000 was a big one.
Besides being the year everyone was celebrating the new millennium it was one filled with
uncertainty all because of Y2K.
Y2K, for those too young to remember, was a computer bug that was anticipated years
before 2000 actually arrived.
The fear was that software that represented the year with the last two digits would switch
over from 99 to 00, and the computer programs wouldn't be able to tell the difference
between the year 1900 and the year 2000.
This would, in theory, cause all sorts of problems with all sorts of computer systems.
The issue wasn't worth ignoring, but with years to update software as needed to avoid
the bug, the problem wasn't much of an actual problem.
However that didn't prevent months of ever increasing fear over what potential apocalyptic
catastrophe we would meet when the clock struck 12 that New Years Eve.
Some people were taking survival courses, stockpiling supplies, and even preparing bunkers.
They genuinely thought this Y2K bug might trigger the downfall of civilization.
The world wasn't going to end on January 1st, 2000, but Disney wasn't going to chance it.
For their own Y2K prep, Disney planned to shut down select attractions in Disney between
11:45pm and 12:15am that would be especially troublesome to evacuate in the event something
did happen.
The list included Peter Pan's Flight which had guests suspended in hanging ride vehicles
and both Rocket Rods in Tomorrowland and the Haunted Mansion in New Orleans Square which
both required elevators to access.
According to an LA Times article the planning went beyond just shutting down attractions
for a while.
All of the departments at the park had access to cell phones in the event that landlines
malfunctioned at midnight.
In the event those cell phones stopped working, they also were outfitted with walkie talkies.
And if the walkie talkies failed?
Well, each department had designated runners who would be responsible for hand delivering
messages during a coordinated evacuation.
All departments were also provided with Y2K labeled flashlights in the event of a wide
scale power outage.
Now looking back, those precautions might have seemed like overkill, but considering
the competition, it wasn't so bad.
Over in California for instance, Universal Studios and Knott's Berry Farm decided to
play it safe and close rides for the night at 11:45pm.
Magic Mountain closed its doors entirely at 8pm that night.
Obviously when January 1st, 2000 arrived, nothing really happened.
There were a few isolated reports of software issues, but nothing that wasn't eventually
fixed.
Planes weren't falling from the sky, power grids weren't going offline, and thankfully,
the only thing going on at the Disney Parks was celebration.
-------------------------------------------
How to Make Amazing Pinball Game Machine from Cardboard - Duration: 5:12.
Thanks for watching
Hope you have a great time
Please, like, comment and subscribe for more!!
-------------------------------------------
Cringe-Worthy Questions Reporters Asked During Interviews - Duration: 4:39.
There are a ton of mundane celebrity interviews out there, but every now and then you get
one that really stands out.
In the following cases, what really separates the wheat from the chaff are the bizarre questions
reporters felt the need to pose, and the equally awkward (and sometimes awesome) reactions
those questions garnered.
Check out which ill-conceived interview questions had us cringing in our seats.
Types of trees
When Barbara Walters interviewed screen legend Katharine Hepburn in 1981, the actress compared
her own strong-willed personality to that of a tree for its sturdiness.
"I'm a very strong -- I've become a sort of… you know, tree."
"You know a tree or something."
And instead of letting that odd analogy go, Walters pressed on with it and asked:
"What kind of a tree are you?
If you think you're a tree?"
"I hope I'm not an elm."
Walters has never really lived down the bizarre question, although she's certainly got some
other highlights for her blooper reel:
"Is it all you?"
"Well, I can't show you."
"Why didn't you have your nose fixed?
Everybody had to say you should have your nose fixed?
How did you know it was going to be right?"
Literacy and exhaustion
Model-turned-actress Cara Delevingne was at the receiving end of a particularly awkward
line of questioning when, in July 2015, she appeared on a local Sacramento morning show
to promote her film Paper Towns.
Things started out on a sour note when she was asked whether she'd ever read John Green's
book upon which the film was based.
"The book is taught at a lot of high schools.
Did you get a chance to read it or do you even have time to read?"
Delevingne was obviously annoyed or offended by the question because she snapped back:
"No, I never read the book or the script actually, I kind of winged it."
Things continued to disintegrate with the interview, to the point that one of the anchors
made the comment:
"I saw you in London talking a couple of weeks ago on TV and you seemed a lot more excited
about it than you do right now.
Are you just exhausted?"
Delevingne was visibly taken aback by the question and insisted she was still jazzed
about the movie, but that didn't end the awkwardness.
"You do seem a bit irritated.
Perhaps it's just us."
"Yeah, I think it's just you."
Super Bowl switcheroo
Although there are a lot of Hollywood doppelgangers who don't mind getting mistaken for one another,
Samuel L. Jackson will not tolerate being mistaken for Laurence Fishburne.
A Los Angeles news reporter made the grave mistake of asking Jackson:
"That Super Bowl commercial, did you get a lot of reaction to thatSuper Bowl commercial?"
The reporter was obviously referring to the year's buzzy Matrix-themed car commercial,
but Jackson didn't star in that one.
"What Super Bowl commercial?"
Once the reporter realized his mistake, Jackson lashed back anyway, saying:
"See, what, you're as crazy as the people on Twitter."
The reporter owned up to the mistake and apologized for the error, but Jackson wasn't done dunking
him just yet.
"We don't all look alike!
We may be all black and famous but we don't all look alike."
"You're the entertainment reporter for this station and you don't know the difference
between me and Laurence Fishburne!?"
Jackson went on to list all of the commercials that he and other black actors had done to
both rub the anchor's nose in his mistake and better prepare him for next time.
Fleeting moment
Heiress and reality show veteran Paris Hilton was on the receiving end of an offensive question
during her 2011 appearance on Good Morning America when the correspondent asked her:
"Do you ever worry about your moment having passed?"
Hilton had already been eyeing the exit sign and decided to walk off camera after basically
being asked if her 15 minutes were over.
To her credit, though, she did return with a well-rehearsed rebuke:
"I've been in this business for 15 years now, so it's been a very long time, and just like
any other businessperson or someone in the industry, it's always important to reinvent
yourself and come up with new projects."
Shutting it down
British actor Tom Hardy has played a number of tougher-than-nails characters on screen.
And he definitely wasn't taking any guff in 2015 when a reporter at the Toronto Film Festival
discussed how is character in Legend contrasted with his own public persona.
"In the film your character Ronnie is very open about his sexuality, but given interviews
you've done in the past your own sexuality seems a bit more ambiguous."
Hardy's face clearly indicated that this wasn't a desirable direction for the question, but
the reporter then ventured to ask him:
"Do you find it hard for celebrities to talk to media about their sexuality?"
This time, it was director Brian Helgeland's face that said it all, and Hardy was not one
bit amused.
"What on earth are you on about?"
Hardy then asked the reporter to repeat his question, and things got even more awkward.
"I don't find it difficult for celebrities to talk about their sexuality… are you asking
me about my sexuality?"
"Um, sure."
"Why?"
You could cut the tension with a knife.
Thanks for watching!
Click the Nicki Swift icon to subscribe to our YouTube channel.
Plus check out all this cool stuff we know you'll love, too!
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The Kate by Tiny House Building Company | Gorgeous Small House Design - Duration: 2:06.
The Kate by Tiny House Building Company
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Is salt REALLY as unhealthy as you've been led to believe? - Duration: 10:31.
Hey everybody out there.
Coach Dan Long with Get Lean in 12.
And I have some awesome things I want
to go over about salt today.
So if you're curious about salt and what's good for you
and what's bad for you, you got to listen up.
Now, you're going to see a link up here.
Don't tap that link just yet.
I'm going to have something for you at the end of this video.
But first I want to talk about salt.
What salt is the best salt?
Now today, I have 2 different salts
here that I just wanted to show you and compare.
This is a sea salt and this is a Himalayan salt.
I know, it matches my shirt.
Did I do that on purpose?
Probably.
So the Himalayan salt is awesome for you.
Sea salt is awesome for you.
But what about table salt?
So minerals are absolutely vital in our diet.
They have to be in our diet, otherwise,
our body is not working at optimal level and pace.
Correct?
Our bodies can't produce all the minerals
that we need in function to be able to function properly.
So, how do we get those minerals into our body?
We have to eat them.
And so by eating minerals, like salt--
the correct salt-- it actually gives you a ton of benefits.
So let me give you some of those benefits now.
Salt regulates water content in the body and brain and blood
pressure.
Obviously, too much can cause higher blood pressure,
so we have to monitor that.
In particular, sodium chloride and potassium chloride
is a benefit that our body needs.
It also promotes healthy blood sugar levels.
So if you're not getting enough salt in your diet,
it's important to make sure that you're
incorporating these minerals because it
regulates blood sugar levels.
Also, various salts are important for bone
strengthening including calcium.
So if you want strong bones, you've
got to make sure you're getting your salt content.
It's also necessary for muscle contraction, in particular,
calcium.
That's how the muscles contract.
It's very important that you get that.
Promotes nutrient absorption in the intestine.
Many salt ions are cofactors that activate enzymes.
These include magnesium, manganese, iron, cobalt,
nickel, copper, and zinc.
All essential minerals that we need for proper body function.
Salts promote transport of substrates,
like sodium, potassium, calcium, and magnesium,
which is important for the function
of the cells in the body.
Important-- the minerals in our diet
are essential for a variety of bodily functions.
So, if you don't have any of this kind of salt--
that today that's why I wanted to bring this up to you.
This is important that you incorporate
some of these salts in your diet and not just
table salt, which I'm going to talk about here in a second.
Table salt-- man, I can't shake my head
enough about table salt. Trust me,
when I tell you what we have going on the table salt
these days, you're not going to want to ever consume that ever
again.
1 of the best ways to obtain these vital minerals
into your diet is to eat the salt. Now,
you've got to eat the correct salt,
and that's why I'm introducing you to these salts today.
Now, refined salt, you can find in the grocery store.
It's pretty common.
It's all over inside grocery stores.
Morton's one of the biggest sellers of this type of salt.
Salt in its natural form is referred to as unrefined salt.
So unrefined salt is a Himalayan salt,
which is coming from a rock.
And also, sea salt, which comes from what?
The sea.
Correct.
All right.
So unrefined salt has not been altered by humans,
so remember that.
Unrefined salt-- not been altered by humans.
This kind of salt contains many different minerals and elements
that are essential for our bodies.
For example, unrefined salt, such as Himalayan salt,
which I'm going to go ahead and show you some of this.
So I'm going to pour some of this in here so you can see.
It's such a beautiful salt. Now I'm
going to pour a little bit in my hand here, so you can see.
See how coarse it is, and see how the color is like a pink.
It's like a pink.
It's Himalayan salt. It's beautiful.
It tastes amazing, and it has a ton of essential minerals
that you need and I need for our diet.
So now, Himalayan salt is over 250 million years old
that has been exposed to high tectonic pressure surrounded
by ice and snow high in the mountains.
That's what Himalayan salt is, i.e.
why the name Himalayan.
When observed under a microscope,
this salt has a perfect crystalline structure,
which you can see.
Again, 1 more time.
Let me just show you here.
All right.
I hope you can see that.
Yeah, see that.
It's an amazing structure.
This salt is incredible for you.
It is believed to be the highest grade salt available.
Now, sea salt is also unrefined, and it's also amazing too.
But Himalayan salt-- that's why I want to bring this up--
you should be consuming 1 of the 2
of these kind of salts and not table salt.
And we're going to go over that in a second.
This salt has an unlimited shelf life,
and doesn't require any pouring agents.
That's why it's better than table salt.
Now, this is contrasted with refined salt. Now,
refined salt, remember what I said,
that's been touched by humans from the color to the shape
to the texture.
It contains 97.5% sodium.
This is refined salt with the rest being added chemicals that
are inside your salts, all of which,
you do not want in your food.
That's why you have to consume some good salts instead
of those salts, otherwise, it actually
can kill us faster than an amazing Himalayan salt.
Now, why is salt refined anyway?
Why do we even do that to such an amazing salt?
Why would we even waste our time?
Now sea salt, let me show you this by the way.
Let me put some of this in here.
Again, this is coarse and you can see it's also coarse,
and it also has a texture.
This is sea salt. Sea salt is also another amazing salt.
But, why do we refine salt anyways?
1 reason, for shelf life, just like pasteurized foods and that
stay on certain shelf lives forever.
And you also have foods that sit out there,
and they're processed.
And they sit there forever.
It's the same thing when it goes for salt. Salt, when it's
refined is done for shelf life.
Refined salt having all of its minerals removed.
It's purified.
It ensures a long shelf life.
In fact, refined salt in the grocery store
will stay on the shelf forever to maximize profits.
That's the only reason why salt is refined.
Manufacturers believe that all white salt product will
look cleaner to the consumer, which is us,
and, therefore, increases their sales,
which gives them more money.
Now, I don't know about you, I don't
want to give them more money so I can die faster.
So refined salt, it's bleached.
And that's why it's white.
It's bleached, in order to obtain that color.
Iodine is added to refine the salt
to prevent the swelling of the thyroid.
But there is insufficient iodine in refined salt
to prevent thyroid illnesses or to provide
for the body's iodine needs.
So now, if you have a choice, what are you going to choose?
I don't even have refined salt here
because I don't carry that in this household.
My wife and I are against it totally.
And I hope you are too because we
don't want you spending money on something
that's not good for you.
Sea salt is great.
So if you have a choice, it's simple.
After what I just said, it's simple to me.
You have Himalayan salt and sea salt. Himalayan being they say,
the best salt on the planet, in the world.
And then, sea salt, also another great salt that
provides up to 80 minerals--
up to 80 minerals.
Refined salt-- pretty much 0.
So cut clear.
Very cut clear here.
Himalayan salt. Sea salt. You know
which 1 that you should get.
Now, just for being here, I got a link up here.
Now part of your diet is part of exercise.
You need to be dieting and exercise 24/7.
And you should be exercising at least 12 minutes per day.
And that's why I have something specific for you
for being here today.
Because I know all the people from around the world,
like yourself, that are watching that
get this great information, you need
a 12-minute bursting exercise program
to go with your diet and your salt
because you want to live long.
And if you want to live long, I have the specific thing
you need right up here at this link.
You can click that link.
When you click that link, you're going
to go to product called the Over 40 Ab Solution.
Now, my buddy Sean and I and our wives
use this specific protocol.
It's only 12 minutes to get the bodies that we desire,
and we're all over the age of 40.
As a matter of fact, Sean's wife is 57.
My wife, she's turning 41.
And I'm actually 44, and Sean's 47.
Now we all stay fit and, we all help tons of people
around the world like yourself.
And we wanted to give you this link
up here that you can click now.
It will take you to the Over 40 Ab Solution
that you can have a proper diet and add
in just 12 minutes of exercise 3 to 5 times a week.
And guess what will happen?
You will get the body that you desire.
Now, tell me who you are.
Say hi.
Put some comments down below.
If you want like, give me a heart, or share.
I would love to see you on the next video.
And I want to thank you again for being here with me today.
I am Dan Long with Get Lean in 12.
And keep going strong.
-------------------------------------------
Post Malone - I Fall Apart (Perto & Made By Tsuki Remix) [Lyrics] - Duration: 3:05.
♪ She told me that I'm not enough ♪
♪ And she left me with a broken heart ♪
♪ She fooled me twice and it's all my fault ♪
♪ She cut too deep, now she left me scarred ♪
♪ Now there's too many thoughts goin' through my brain ♪
♪ And now I'm takin' these shots like it's novacane ♪
♪ (Yeah) ♪
♪ Oooh, I fall apart ♪
♪ Down to my core ♪
♪ Oooh, I fall apart ♪
♪ Down to my core ♪
♪ (Yeah) ♪
♪ Oooh, I fall apart ♪
♪ Down to my core ♪
♪ Oooh, didn't know it before ♪
♪ Surprised when you caught me off guard ♪
♪ Hard as damn jewelry I bought ♪
♪ You was my shorty, I thought ♪
♪ Everybody told me so ♪
♪ Feelin' like I sold my soul ♪
♪ Devil in the form of a whore ♪
♪ Devil in the form of a whore ♪
♪ You said it ♪
♪ No you said it ♪
♪ No you said it ♪
♪ We'd be together ♪
♪ (Oooh) ♪
♪ (Yeah) ♪
♪ Ice keep droppin' and the drink keep flowin' ♪
♪ Try to brush it off but it keep on goin' ♪
♪ All these scars, can't help from showin' ♪
♪ Whippin' in the foreign and the tears keep blowin', yeah ♪
♪ Oooh, I fall apart ♪
♪ Down to my core ♪
♪ Oooh, I fall apart ♪
♪ Down to my core ♪
♪ Oooh, didn't know it before ♪
♪ Surprised when you caught me off guard ♪
♪ Hard as damn jewelry I bought ♪
♪ You was my shorty, I thought ♪
-------------------------------------------
НОВОГОДНИЙ РЕЙД | Cam Pranks — Пранки c камерами - Duration: 8:50. For more infomation >> НОВОГОДНИЙ РЕЙД | Cam Pranks — Пранки c камерами - Duration: 8:50. -------------------------------------------
Hah Hah! POTUS Trump Trolls Global Warming Zealots as Record Fre - Duration: 2:05.
Hah-Hah!
POTUS Trump Trolls Global Warming Zealots as Record Freezing Temps Grip US
The United States is gripped in a freeze as record chill temps ravage parts of the country.
President Trump trolled global warming zealots Thursday on Twitter and celebrated the fact
that we aren�t paying trillions of dollars to fight the hoax.
Please president Trump, keep tweeting!
Trump tweeted Thursday, �In the East, it could be the COLDEST New Year�s Eve on record.
Perhaps we could use a little bit of that good old Global Warming that our Country,
but not other countries, was going to pay TRILLIONS OF DOLLARS to protect against.
Bundle up!�
President Trump has accurately called Climate Change a hoax.
Climate Change is a global redistribution of wealth based on junk science.
The U.S. should NEVER have to answer to foreign bureaucrats; we are a sovereign nation.
Trump kept a campaign promise when he pulled out of the Paris Climate Accord which would
have robbed Americans of wealth and freedom.
The US is getting blasted with record freezing temps.
Forecasters are saying we may see record freezing temps in the Northeast for New Years Eve.
We sure could use some of that global warming!
News of the freeze dominated headlines on the Drudge Report.
-------------------------------------------
Rhonda Shear "Ahh Dreams" Hooded Poncho - Duration: 6:28.
For more infomation >> Rhonda Shear "Ahh Dreams" Hooded Poncho - Duration: 6:28. -------------------------------------------
DNA sequencing by Maxam Gilbert method - Duration: 18:02.
Hello friends today in this video I am going to discuss about the DNA sequencing by Maxim Gilbert method
Okay, so at first
What is DNA sequencing DNA sequencing is a process which is used to know
the sequence or order of nucleotides
present in a DNA molecule, okay, so now this DNA sequencing technique was discovered by
maxam and Gilbert
And that's why this DNA sequencing technique is called the maxam
Gilbert DNA sequencing
Which is also known as the chemical degradation method because this DNA sequencing technique is
based on the chemical degradation
that means some chemical reagents are required to cut or
degrade the DNA molecule at a specific nucleotide
And that is the most important for this DNA sequencing and thats why this is called the chemical degradation
Method okay, so now let's talk about the whole DNA sequencing method step by step
Okay, so the first step is end level
End leveling means the labeling of DNA molecule by radioactive
Isotope at the both End of the DNA molecule
Okay, so now
Suppose. This is a DNA molecule
This is the DNA molecule which we want to sequence okay
And we all know that at the 5' position the phosphate group is present which is normal phosphate not
Radioactively labeled phosphate, okay, so now we have to replace this normal phosphate group at the 5 prime position
with the
Radioactive isotope of phosphate that is P 32 okay
So for that reason at first we have to remove this
Normal phosphate by the alkaline phosphatase which removed this
Normal phosphate, and then we get the dephosphorylated DNA molecule
so now we have to add the
radioactive phosphate at the 5 prime end ok and
Is done by polynucleotide kinase at the presence of ATP which
contains the radioactive P 32
Okay
So now this polynucleotide kinase add the radioactive p 32 from the ATP
to the 5' end of the both strand of DNA
Okay, so now we get the end leveled DNA
Molecule okay, so now the problem is for this DNA sequencing we only require
One site only require the labeling at the one site not in both site
Okay
So for that reason we have to cut this DNA molecule
Into two fragments and take only one fragment for the further experiment, okay?
so for this cutting for this cut we have to
Treat with restriction enzyme so the next step is restriction enzyme digestion
So after treatment after treating with the restriction enzyme we get
the two fragment of DNA
molecule okay, so now in this case another problem is
if the if the one fragment is very much smaller than the other fragment then we have to
discard the smaller fragment and take the larger fragment for the further experiment, but if the if these
Fragments are more or less equal
Not not they're unequal but more or less same in size
then we have to take both the fragments and
sequence separately okay so for this experiment suppose we get a
Very small fragment and very large fragment, okay?
So we have to discard the small fragment and take the larger fragment for the further experiment, okay
So now we get the larger fragment and now the another problem in this DNA sequencing is
we get we
required only
The single stranded radiolabeled
DNA fragment, okay so for that reason
We have to generate this double-stranded DNA into single standard form so now the next step is
denaturation
so after denaturation we get the
Single-stranded form and we have to isolate those single standard form that contain the five prime
five prime phosphate that is
Radioactively-labeled okay because this is required for the detection of the DNA molecule in the
autoradiography, okay
So now we get the end lebeled single-stranded DNA and now we have to perform the chemical degradation
Which is the most important of this DNA sequencing okay now
I will discuss about the most important step of this DNA sequencing that is the chemical degradation, okay
so still now we get the labeled single-stranded DNA which we want to sequence and
Here suppose. This is the whole sequence of this single-stranded DNA
Now we have to take multiple copies of this single-stranded DNA
Into this four reaction mixture tubes that is the g-tube, a+g tube
C + T 2 tube and C tube and
each of these reaction mixture tubes contain some different chemical reagents which
Specifically cut the DNA at specific
nucleotide base, okay
So for example in g tube all the DNA will be cut at the guanine base and in a+g tube
all the DNA will be cut
in both adenine and guanine
base and in
C+T tube all the DNA will be cut at cytosine and thymine base and in C tube all the DNA will be cut at
The cytosine base, okay
but remember one thing that
The reaction mixtures are made in such a way that only one cut will occur per DNA that means
If there have multiple
Copies of the DNA of this DNA into suppose the A+ G tube so there are multiple
Adenine and guanine base are present and all this DNA suppose. This is a
This is G. This is a this is G ok so if a chemical reagent
randomly cut
Suppose if we randomly cut in this
adenine base
Then it will never cut in other any base
Because the only one cut will be occurred per DNA suppose in this DNA
molecule the
reagent randomly cut in this
Guanine base it will never cut in other base okay?
Now I will discuss about the whole mechanism of this cleavage action ok so at first in G
Tube as a reagent we use dimethyl sulfate which
Specifically modified the guanine base by adding methyl group at the guanine base so for example suppose
This is our DNA sequence and here the guanine is present so when we add diethyl sulfate
Then it will modify the guanine base, and then it will release that guanine base not the nucleotide okay
And then when we add piperidine which is another reagent, then it will
cut the phosphodiester bond
And then remove this nucleotide modified nucleotide ok so in this way cleavage will be occur okay
So now here in this g tube only the g base will be cut so
Here we can see that only 2 G is present okay
so we will get 2
fragments for this G and this G when this g will be removed then when it then we will get only the phosphate group and
When this g will be removed we will get this fragment. Okay, so in this G tube. We will get
Two fragments all together two fragments we will get
Okay, and in this tube that is a plus G tube. Also. We use
Dimethyl sulfate as the reagent but in presence of formic acid so in this condition
dimethyl sulfate
Specifically modified all the adenine base, but occasionally modified guanine base also
Okay, so in this case
We will
cut we will modify the adenine and also guanine base by the
Dimethyl sulfate and then piperidine will come and cut and remove that nucleotide. Okay, so here
We can see that all together one two three
four five six all together six
base are present and so we will get six fragment in this a plus g tube because
when this g will be removed we will get only the
This fragment and when a will be removed then we will get this fragment when this a will be removed
Then we will get this fragment and so on this fragment this G will be removed
We will get this fragment so in this way all together we get
six DNA fragments in this a plus G tube and in c+ t tube we will
We use
Hydrazine as a reagent and this hydrazine
specifically cut specifically break the
specifically break the ring structure of this cytosine and thymine base and in this way this hydrazine modify the
cytosine and thymine base and then piperidine will come and
recognize that
modified base, and then they cut and removes that
Nucleotide okay so in this way
the
DNA will be cut in this C plus T tube and here we can see that all together
one two three
Four five six bases six C and E bases are present in this DNA so there will be six
We will get six fragment
because when
This T will be removed
We will get this fragment when this t will be removed we will get this fragment and so on okay and this C tube
We use hydrazine also use hydrazine
but in 2Ml NaCl and this in this condition the
this condition the hydrazine will remove will modify only the
Cytosine based not the thymine base and then piperidine will come and cut and remove that cytosine base
base containing nucleotide, okay, so now so now here. We can see that all together
Two cytosine bases are present in that DNA so when this cytosine will be removed
We'll get this fragment when these cytosine will be remove we will get that fragment. Okay, so all together we will get
Two fragment and here we get six fragment
So this is the whole mechanism of this chemical degradation after chemical degradation is done now
We have to separate the DNA fragments which are present in those tubes
According to their size by high resolution gel electrophoresis, okay?
So and remember that the DNA fragments which are present in those tubes
will run in the different Lane, okay, and
the larger DNA fragment will migrate less in the gel and present on the top of the gel and the
lower or the smaller DNA fragment will migrate more in the
gel and present on the bottom of the gel okay, so now after gel electrophoresis is done
Then we have to see these bands by autoradiography, okay, so after autoradiography
Then we can see those bands
And then we have to determine the sequence or order of nucleotides which are present in this DNA
Okay, so now we know that our DNA
fragment is 12
Nucleotide long so the largest nucleotide long so the largest DNA fragment what we can get in this
Gel that is the 12 nucleotide long DNA fragment and that
12 nucleotide DNA fragment we get in the c tube in prominent and in high amount and in c-plus
T tube in low amount so from this we can say that
The last nucleotide of this 12 nucleotide DNA fragments will be C not
T because in c tube it will get we will get larger amount of
DNA and c+t tube we get only small amounts that will be that for the C, okay, so
as in the c tube the DNA will be cut in the C so we get
the bands in c tube which
Determined that the last nucleotide will be the 12th nucleotide will be the C
Okay, so the next is
11 nucleotides DNA fragments 11 nucleotides DNA fragment
We get in the C plus T tube, but not in the C tube, so it will be T
not c because we don't get any band in the c so the
11th number nucleotide will be the T. Okay will be the T and the
Next one is the same so this is also T
and the 9th
Nucleotide nine nucleotides DNA fragment we get in the A plus G tube
Okay, so as we don't get any bands in G tube
So we can conclude that there the last
last nucleotide of this nine nucleotides DNA fragment will be A not G because
They are in G Tube. No bands We get okay so the ninth
so the ninth nucleotide will be a
So the ninth nucleotide will be a and next
One is also same that is the a and the seven
Seven nucleotide the seven nucleotides DNA fragment we get in high among in G
But in low amount in A plus G so from this we can conclude that the seventh number nucleotide will be
G okay not a because here we get the bands
for g not a in low amount okay
So in this way, we can sequence all the nucleotides
and then we have to read this sequence from the bottom to top because the
First nucleotide is the G that is G and the last nucleotide 12 new is C
Okay,
this will be the
5 prime and this will be the 3 prime ok
So in this way, we can sequence we can determine the sequence of the DNA
Fragment and now the whole process is at first we have to label
we have to end leveling have to do end leveling that is the leveling the whole DNA in
both the end by radioactive isotope, then we have to cut the
labeled DNA in middle by restriction enzyme so that we can get the get the
radioactive isotope or labeled DNA at only one site
And then we have to do the double-stranded DNA into labeled single standard form and then we have to perform chemical
chemical degradation
in the
tubes reaction tubes where the
specific reagents will cut the DNA fragments will cut the DNA fragments at
Specific nucleotide base ok and then gel electrophoresis is done to separate the DNA
Different sized DNA fragments, and then we can see those bands by autoradiography
And then we have to determine the sequence of this
nucleotides ok so this is the whole process of Maxam and Gilbert DNA sequencing ok now there have some limitations or
disadvantages of this maxim Gilbert DNA sequencing the main two disadvantage of this
DNA sequencing the first one is in this DNA sequencing we use radioactivity and
Also be used toxic chemicals
And that was risky and the second disadvantage of this
DNA sequencing that is by this DNA sequencing we can sequence only up to
500 base pair okay, and that is the main disadvantage of this Maxam and Gilbert DNA sequencing, okay, thank you for watching this video
-------------------------------------------
Forbes on Fox 12/30/17 11AM | December 30, 2017 Breaking News - Duration: 34:01.
For more infomation >> Forbes on Fox 12/30/17 11AM | December 30, 2017 Breaking News - Duration: 34:01. -------------------------------------------
Cavuto on Business 12/30/17 10AM | December 30, 2017 Breaking News - Duration: 34:26.
For more infomation >> Cavuto on Business 12/30/17 10AM | December 30, 2017 Breaking News - Duration: 34:26. -------------------------------------------
Cavuto on Business 12/30/17 11AM | December 30, 2017 Breaking News - Duration: 40:37.
For more infomation >> Cavuto on Business 12/30/17 11AM | December 30, 2017 Breaking News - Duration: 40:37. -------------------------------------------
Heather Fell's Cérvelo P2 | GTN's Presenter Pro Bike - Duration: 3:15.
- Well, this is my Cervelo P2, which I had the chance
to ride on a shoot in Mallorca.
I also got to race on it in Chattanooga
at the World Championships.
(relaxed electronic music)
I actually only got on a time-trial bike
for the first earlier this year, and rode another bike
for a couple of months.
I then got on this one,
and I was amazed at how smooth it felt and how easy to ride,
and it's also pretty slick and understated.
I genuinely really love this bike.
It's a 51-inch frame, and attached to that,
we've got profile-designed T2 Wing Aero base bars,
and then the Profile-designed T4 Plus Aero bars.
On the end of those, we've got the shifters
for the Shimano Ultegra DI2.
So I'm using Shimano Ultegra front and rear Derailleurs
then Shimano Dura-Ace chain ring at 52/36,
which was quite helpful for getting up the big climb
in Chattanooga and then an 11/28 cassette on the back.
As you can see, it's using the Quarq Power Metre,
and it's the very first time I've ever ridden with power,
so racing with it was quite a new experience
obviously I didn't need to look at the numbers
going up the hill but for the rest of it,
it was really interesting,
and I managed to stick to my plan pretty well.
I've got 172.5 length cranks on the end of those,
the Shimano Ultegra pedals.
As you can see, I put Shimano 105 rim brakes
on the front, and the back,
I'm using the Vision Metron wheels,
got the 55SL on the front, and the 81SL on the back,
and then on those, running the Continental Gran Prix tyres,
at 25 MM wide, and for Chattanooga,
I put in a pressure of 190
because the roads were a little bit rough in places.
For Cevelo Aero seat posts,
and then on top of the is the ISM Adamo Prologue
split-nosed saddle, and it's actually the first time
I've ever ridden a split nose saddle,
and compared to the time-trial bike
I was on previously, this is so comfortable,
I genuinely feel like I can ride this bike all day
with no discomfort.
As we're talking about comfort,
you can see that I've still got space
left in my position isn't that aggressive,
but being pretty new to time-travel bikes
this year, I very much wanted to be comfortable
and feel confident on the bike
and then be able to have that consistent power
that I needed to target.
Well as you can see since Chattanooga,
I have actually given the bike a bit of a clean,
removed all the extra bits,
but I stored my spares behind the saddle
for the race day, and then I actually use
profile-designed Aero bottoms for my Aero bars at the front,
and then I had a bottlecage on the downtube,
but there's also options on this bike
to have a storage box on the top
for any of you nutrition.
For you weight weenies out there,
it comes in at 8.9 KGs, and just remember though,
there are none of the extras on this at the moment,
but going through my bike for this pro bike
has made me realise how much I love the P2
and I think I should get back out on it,
I just need the weather to get a little bit nicer,
if you've enjoyed this, give it a thumbs up like,
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and if you want to have a look at Mark's pro bike,
click up here, if you want to see me riding this bike
in action, then check out my Chattanooga blog down here.
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