Colombia Next Top Model - Before and After Makeup
-------------------------------------------
La hija de Raúl de Molina le echó a perder su fin de semana - Duration: 2:56. For more infomation >> La hija de Raúl de Molina le echó a perder su fin de semana - Duration: 2:56.-------------------------------------------
En Chivas tendrán que dejarles una foto a sus familias: cuatro partidos en una semana - Duration: 1:05. For more infomation >> En Chivas tendrán que dejarles una foto a sus familias: cuatro partidos en una semana - Duration: 1:05.-------------------------------------------
Snapchat Story: Chinese New Year 2017 in Chinatown, London (Year of the Rooster) - Duration: 2:11.Hello to all the followers of @codigonuevo
My name is Oriol Salvador, I'm in London and I'll bring you to Chinatown to see how they celebrate
Chinese New Year.
Chinese New Year or Spring Festival started last Friday and goes on until February 22nd.
Celebrations in London's Chinatown are mainly TODAY.
What is he doing?, may you ask. Bringing good luck to stores and restaurants in the new year, they told me.
Don't be fooled by souvenirs stores, 'maneki neko' (this cat) is not Chinese,
it's Japanese.
Later we'll go back to Chinatown but now we go to Trafalgar Square where Chinese community
is celebrating the New Year with shows and food stalls.
Access to Trafalgar Square was closed, but we're in now!
Visiting Hong Kong's skyscrappers on a VR experience.
"How can they jump so high?!"
"Are you hungry?"
Find out what animal on Chinese zodiac are you according to your birth date. I'm a dragon.
"Happy Chinese New Year everyone. We are celebrating the Year of the Rooster,
we celebrate everyone born in the year of the Rooster and hope you have lots of prosperity
and good luck in the new year.
Everyone wants to take a picture with this man,
he is the God of fortune and gives away red envelopes
with money inside, to attract
wealth on the new year.
That's it from me from the Chinese New Year in London's Chinatown.
It's being a pleasure to share it with you.
You can find me in other social media as @oriolsalvador
Oh! If you also want to be an ambassador for @codigonuevo Snapchat, get in touch NOW
-------------------------------------------
Vale Saber///Fernando Amate, Cronista de Celaya, la antigua relación con Estados Unidos - Duration: 3:24. For more infomation >> Vale Saber///Fernando Amate, Cronista de Celaya, la antigua relación con Estados Unidos - Duration: 3:24.-------------------------------------------
'Paco' Jémez: "Mis jugadores se dejan los hue… en la cancha" - Duration: 0:14. For more infomation >> 'Paco' Jémez: "Mis jugadores se dejan los hue… en la cancha" - Duration: 0:14.-------------------------------------------
Cecilio Domínguez: "Sé que es un importante, pero yo no veo el número que tengo atrás" - Duration: 1:04. For more infomation >> Cecilio Domínguez: "Sé que es un importante, pero yo no veo el número que tengo atrás" - Duration: 1:04.-------------------------------------------
Guía Saint Seiya Zodiac Brave; Dohko de Libra ♎ - Duration: 2:28. For more infomation >> Guía Saint Seiya Zodiac Brave; Dohko de Libra ♎ - Duration: 2:28.-------------------------------------------
BIKINI STYLER BRAUN | REVUE - Duration: 3:27.Hello guys today this video is about the bikini styler from braun
The package look like this
Is a small styler for bikini is look like a T is very handy
Let me show you guys how it is.
He came with a small bag very nice
Inside you have 2 small brush one is 8mm and the others 5mm
Is for decreased the length of you hair before shaving.
You also have a smaller head for style you eyebrow
also using it for you bikini
And a brush for clean it.
There is stickers for design you bikini
I made this revue because is a month now am using this product and I love it so much
Before I use to shave with razor but I had pimpo and black mark (bad experience)
Then I used remover cream is good but some time is just fucking burning me
Since am using this bikini styler everyting is perfect no pimpo, no burning, no black mark… Also I didn't cut myself with it.
You have to know that you not gonna have same result like when u waxing Is not gonna be soft you gonna have 1mm of hair
I prefer to use this than shaving with a razor and having pimpo
Anytime that im gonna try something nice am gonna share with you guys
For all that using razor and scaring about the hair remover cream I advise you to you use this bikini styler is very very nice I bougth it 15£
You can avoid having pimpo or black mark for people that having this problem
So that it sending you so much kiss and love
-------------------------------------------
Amor Bravío | Capítulo 80 - Duration: 4:20. For more infomation >> Amor Bravío | Capítulo 80 - Duration: 4:20.-------------------------------------------
Isabel Pantoja en el Hormiguero Enero 30 - 2017 parte 1 - Duration: 10:04. For more infomation >> Isabel Pantoja en el Hormiguero Enero 30 - 2017 parte 1 - Duration: 10:04.-------------------------------------------
Kara Sevda 54//Fragmento 2 - Duration: 0:43. For more infomation >> Kara Sevda 54//Fragmento 2 - Duration: 0:43.-------------------------------------------
Franela teñida y estampada EN CASA! // Tie dyed and printed tee AT HOME! - Craft Nation - Duration: 4:54.Crepe paper
Hot water
Dry for 2 hours
Dry in the sun
Fabric
Let it dry
Fix the edges with a brush
-------------------------------------------
Artist Zayn Malik Live For more infomation >> Artist Zayn Malik Live-------------------------------------------
Choosing Love After Tragedy... For more infomation >> Choosing Love After Tragedy...-------------------------------------------
Stary trzepak na omegle u Cinkowsky :D - Duration: 6:10. For more infomation >> Stary trzepak na omegle u Cinkowsky :D - Duration: 6:10.-------------------------------------------
Phillips, Craig & Dean Come Now Is The Time To Worship Cover With Unconditional Love Xo Enjoy :D ! - Duration: 2:52.Come, now is the time to worship.
Come, now is the time to give your heart.
Come, just as you are, to worship.
Come, just as you are, before your God.
Come.
One day every tongue will confess You are God.
One day every knee will bow.
Still the greatest treasure remains for those Who gladly choose you now.
Come, now is the time to worship.
Come, now is the time to give your heart.
Oh, come.
Just as you are to worship.
Come just as you are before your God.
Come.
One day every tongue will confess You are God.
One day every knee will bow.
Still the greatest treasure remains for those who gladly choose you now.
[Repeat]
Come, now is the time to worship.
Come, now is the time to give your heart.
Come, just as you are to worship.
Come, just as you are before your God.
Come.
Oh, come.
Oh, come.
Oh, come.
Worship the Lord.
Oh, come.
Come, come, come...
-------------------------------------------
The Space Between Us For more infomation >> The Space Between Us-------------------------------------------
Build Your Dreams With Lego For more infomation >> Build Your Dreams With Lego-------------------------------------------
Man hospitalized after crash involving multiple semis on I-65 - Duration: 0:34.3
THE NORTHBOUND LANES OF I-
65 WERE SHUT DOWN AFTER A
WRECK INVOLVING THREE SEMIS
BLOCKED THE ROADWAY.IT
HAPPENED THIS AFTERNOON WHEN A
CAR WITH A FLAT TIRE IN THE
CONSTRUCTION ZONE WAS TRYING
TO PULL OVER JUST PAST THE
STATE ROAD 25 EXIT.TWO SEMIS
WERE FOLLOWING THE CAR WHEN A
THIRD SEMI CRASHED INTO THE
BACK OF THE SECOND SEMI,
CAUSING A CHAIN REACTION.THE
DRIVER OF THE SEMI THAT CAUSED
THE WRECK WAS PINNED INSIDE
HIS TRUCK AND LATER TAKEN TO
I-U HEALTH ARNETT HOSPITAL
WITH LEG INJURIES.THE
NORTHBOUND LANES WERE CLOSED
-------------------------------------------
Drake Hosting Half Naked Chicks on Super Bowl Weekend! I TMZ Live - Duration: 2:40.CHARLES: MOVING ON TO THE SUPER
BOWL WHERE DRAKE IS PLANNING
SORT OF AN ALL-STAR EVENT THAT
HAS NOTHING TO DO WITH PLAYERS
BUT A BEST OF YOUR KNOWLEDGE OF
PARTS LEADING -- A BUNCH OF
PLAYERS LEADING UP TO IT.
WE FOUND OUT AND IT IS CALLED
THE BALLET AND HE IS DOING THIS
-- HE IS CO-HOSTING THE EVENTS
WITH JAZZ PRINCE THE MUSIC
EXECUTIVE WHO IS A HOUSTON
NATIVE WHO DISCOVERED DRAKE.
SO IT IS THE BALLET.
THERE WILL BE DANCERS BUT I'M
PRETTY SURE THEY ARE NOT BALLET.
>> YOU KNOW HOW ACE OF DIAMONDS
IS WITH THE BODY SUIT AND
SHOWING BOOTY.
IT WILL BE NICE AND TASTEFUL.
HARVEY: IT IS LIKE A POP-UP
STRIP CLUB WITHOUT THE STRIP.
>> HE'S BEEN PLAYING THAT A LONG
TIME.
HIS PLAN IS TO OPEN A STRIP CLUB
IN HOUSTON SO HE KICKS IT OFF,
WHAT BETTER WEEKEND.
CHARLES: NEXT WEEK.
>> YES, SORRY.
HE WILL HAVE LITTLE WAYNE AND
JEEZY, ODELL BECKHAM.
GOOCHY MAY.
HE WILL HAVE QUITE A LINEUP.
>> HE HAS TWO DIFFERENT
CELEBRITIES PER NIGHT AT THE
VENUE FROM THURSDAY TO SUNDAY.
HARVEY: WHERE?
CHARLES: THE MERCHANTS SIT
>> THE MERCY NIGHTCLUB.
HARVEY: THAT IS HARDLY A POP-UP.
CHARLES: IT IS INTERESTING TO
CALL IT THE BALLET.
>> THE DEFINITION OF A POP-UP.
HARVEY: OH, GOD.
CHARLES: THEY ARE GOING TO HAVE
V.I.P. SECTIONS THERE.
WE CAN GET IT.
JUST A THOUSAND BUCKS.
HARVEY: ARE YOU GOING?
>> ARE YOU KIDDING ME?
I'M GOING.
>> I'M CLARISSA.
I WISH I HAD KNOWN ABOUT THERE
BECAUSE I WAS JUST IN DALLAS
THIS PAST WEEK.
I WOULD HAVE POSTPONED MY TRIP
AND TOOK A FLIGHT TO HOUSTON BUT
DRAKE, CALL ME ON THE HOTLINE
BLING.
I GOT YOU UP THERE.
CHARLES: THEY ARE AUDITIONING
NEXT WEEK FOR THE DANCERS.
HARVEY: I'M BABY-SITTING
JUSTICE.
-------------------------------------------
Whites MX Sport problem z poprawnym sygnalizowaniem kapsli i guzików - Duration: 7:09.Whites MX Sport audio problems with bottle caps and some buttons with stock DD coil.
This is not new one video about Whites MX Sport!
I was talk about DD coil issues
Sometimes I'm trying to made some air tests, but not for depth, but for audio response!
Factory DD coil doesn't work good like concentric 950 coil.
Bottle caps are huge challenge.
But I was found that some buttons get iffy trash respond...
Not like in other brands.
Coin and Jewellery or Relics, but is almost the same.
Audio respond is not good. You can't recognize bottle caps cleary, and buttons has broken sound...
Buttons are difficult relics always, but with MX this is huge problem in my opinion!
Relic program is slightly better, you have all metal channel respond, but...
This button syndrome is not for each button... for some...
Now, I know clearly why on USA forums peoples recommended 950 old Whites coil!
ID jumping with buttons, but with bottle caps is almost good... and repeatable!
Ok so I'm going to check it!
Bottle cap
Very little jumping behavior!
On edge you have trash respond!
Button
Old military button
If this kind of button is shallow, respond is broken and jumping to iron level!
Far from coil... good audio.
This is not good
Another button
Ok, I will change tracking to locktrack...
Ok, again
Still jumping
Broken sound
Program Relics
And I'm checking again!
Little better
But is not easy signal
Button
No good
Shallow has unstable ID!
This button has two layers!
Other brands are better if you compare to MX Sport.
Other kind of button
Good without any problems!
Buckle
I should compare it to the 950 concentric coil!
So now I'm not sure... I will miss some buttons with this issue.
PLEASE SUBSCRIBE!
-------------------------------------------
Hyundai ix35 1.6I GDI Active Cool Trekhaak! - Duration: 1:22. For more infomation >> Hyundai ix35 1.6I GDI Active Cool Trekhaak! - Duration: 1:22.-------------------------------------------
il segreto - eliseo gli porta i vestiti - Duration: 0:25. For more infomation >> il segreto - eliseo gli porta i vestiti - Duration: 0:25.-------------------------------------------
Miami Airsoft Fight #2 - Duration: 27:12.Skip to 12:00 to see where the "fight" begins
-------------------------------------------
Hub City Mistakes - 7 Days to Die PS4 (turn on captions for commentary) - Duration: 20:35.My co-op partner and I needed to get back to our original base to pick up about 2000 feathers. The most direct route is through the hub.
We spent a minute getting weapons and ammo together before we head out.
On our way. Notice that neither of us have any medical supplies.
About 4 minutes away from the outer edge of the hub city.
Second mistake: going after this damn rabbit. The meat wasn't a priority and is more than likely what did us in.
Instead of taking responsibility for my part in this farce, I have chosen to place the blame solely on my co-op partner.
Notice how the first growl is covered by a thunderclap. I thought I heard something but wasn't sure.
Pay attention as I casually spin around to investigate what I thought was a noise and discover the awful truth.
:(
A feral quickly joins the fray. He will be around for the next 5 to 7 minutes or so. He even completely disappears at one point and reappears later.
Too close :|
No pic-a-nic basket for you.
It's like he knows when I'm about to pull the trigger so he shambles in the other direction as soon as I do. Bastard.
Yeah, no, why would he be dead?
So far, not so good.
Frantic scrambling for cotton ensues
Shooting him four times in the junk only pissed him off more.
And he vanishes. I'm assuming i just couldn't see him. Either way, he catches up with us later. We'll call him Dickshot.
I think that's Dickshot, but there was another feral lumping about as well so I can't be sure.
(Potentially) Dickshot vanishes again.
I'm looking for his body, I wanted those magnum parts. I figured he had to be dead because I couldn't see him.
Look at us: looting things as if we're going to get out alive.
Is that Dickshot?
I'm convinced it is. We dumped almost three times the ammo into Dickshot and he didn't die, this guy comes along and dies after a modest volley?
RIP Dickshot
I am now attempting to splint my partner's leg.
Buh-buh-buh beeeeee
:( you can literally see the controller jump in their hands when they see the bee
3 ferals closing in from three sides. :(
Ugh. I can see the cop jiggling towards us at the horizon.
Aaaaand the zombear adds the final touches to our death-shit sandwich.
Dickshots brother Cocknibbler shows up and makes good on his name.
Mistakes were made. We fought, we ran, we fought, we died.
I don't know why we stood and fought. Just got caught up I guess.
Don't ever leave the house.
-------------------------------------------
UPDATE Booms UPDATE Booms Under San Diego, CA, the Neutralization of Bouvet Island, ET Battles n - Duration: 11:10.UPDATE - Booms Under San Diego, CA, the Neutralization of Bouvet Island, ET Battles near Alaska,
the Details behind Trump, NESARA and More.
Since the end of 2016, time seems to be moving at an ever-quickening pace.
Adding to this increase is the apparent amount of chaos we see in world events.
Though this chaos may seem unnerving at times, there is said to be an order and significance
to the madness.
The events we have seen as of late with financial reform, a crumbling Cabal, a questionable
American president, and the ongoing process of soft disclosure, tell us that progress
is on the verge of hitting the fast lane.
Over the course of the past few weeks and months, more revelations have come forward
about Cabal activity than ever before.
One by one, the world is kicking off elitist control and setting the standard of the new
world we desire to see.
We may understand that with a world of events unfolding simultaneously, prompt and accurate
coverage can be somewhat of a challenge.
So when Kent Dunn brings intelligence forward, it is important to scrutinize, to be patient
and to keep an open mind.
Here are Kent and Gary with a recent update.
Kent Dunn - Contact Numbers for Fund Distribution; Russian Military Moves, Galactics and Greys
Battling, and Obama Divorce
The story of Bouvet Island is an interesting one which I stumbled upon some time ago.
During the time around mid 2014, it was being reported that a number of hacks to sensitive
locations in the U.S. were taking place.
Upon researching these attacks, I came across a live feed of the various hacking attacks
taking place worldwide.
Within the feed, there was one curious location that seemed out of place.
Most of the attacks occurring either originated from a metropolitan continental location,
or they were directed against one of these locations.
However, there were certain attacks that originated from one remote location in the south Atlantic.
If we look at the Norse Corp live feed, we will no longer see this location as the origin
or target of any of the hacks taking place.
This difference runs consistent with the reports from Kent Dunn about the present status of
Bouvet Island.
However, if we were to rewind time back to the years before this location was neutralized,
we would see attacks similar to those that are still taking place between the U.S. and
various BRICS nations.
Galactic Battles
When we hear of battles taking place between ET groups, a number of ideas may come to mind.
We may wonder what the reasoning is behind why these groups would fight whereas before,
they seemed to be mostly uninvolved with one another.
In order to understand the reasoning for these events we must understand the situation of
Inner Earth.
According to multiple insiders and personalities, the situation of Inner Earth is very similar
to that of surface civilization.
It may be true that these beings have chosen in the past to live more peacefully since
any fighting would lead to massive casualties on both sides.
Within the confines of Inner Earth, there is not much room for violence, not to mention
the fact that most of these groups have been under treaty not to do battle in the skies
above the Earth.
Consequently most of these groups, though they may not have always agreed, they have
made significant efforts to keep the peace�that is until now.
It may be that the recent increase in energetic inflow to the Earth has caused an uprising
of volatility between certain Inner Earth groups.
Due to these increases, those who were already at peace may have become more peaceful, while
those who trended toward negativity may have plotted against one another.
This gradual progression to violence would naturally authorize intervention between the
negative ET groups of Inner Earth and the positive groups here to assist the planet.
This is a likely situation that has unfolded within the confines of our planet.
However, this description only represents one of many possibilities.
Dissatisfaction with Trump
The subject of presidency is not one I enjoy writing about, primarily because it is lower
in vibration than I care to operate at.
However, for the sake of keeping the reading informed, I have written a number of articles
on the present state of affairs.
Along with these articles, I will say this.
It did seem obvious that Donald Trump was not the first pick of the Earth Alliance to
be president.
However, that is what the situation currently requires.
Kent seems to agree with this point as well.
For the time-being, Trump serves as the front man for the positive Earth groups.
Though this may be true, some of his choices do not seem to be in the best interests of
all people.
It is of course our choice as to how we view the situation.
We could either see it as a hopeless pursuit, an assured hope, or we may choose between
the array of possibilities between these.
Yet, no matter how we see this one man we call "President," it is not at all only his
choices, but ours which determine our course.
There have been some decisions coming from the new American establishment that I have
appreciated.
There are also those that I opposed and intend to work against if they are not in the best
interests of humanity.
Whatever the situation, it is important to know that every perceived challenge actually
represents an opportunity for growth, and for monumental improvement of our planetary
situation.
Whenever we encounter potential problems, we must remember that these problems represent
a chance to alter reality for the sake of our future.
Without exaggerating, our destiny can turn on a dime if we are willing do the work necessary
to accomplish the change.
Along with our efforts, there is one other step we may take in order to create a better
world.
There are reported to be numerous benevolent ET groups waiting to assist us in our progress
toward freedom.
Many of these groups have been discussed in past updates as well as by numerous whistleblowers.
These Galactics are waiting to given assistance, but there is a catch.
This is that we have to know what our need is and ask specifically for assistance in
meeting the need.
(This is how these beings respect our free will.)
As I have said before, this is not a prayer to a superior deity figure, but a simple request
to our off-planet equals.
Speaking from experience, any positive request can have amazing effects.
Disclosure and ET Transports
Perhaps the most exciting subjects to discuss are those of disclosure and the first mass
contact Earth has seen for thousands of years.
It is difficult to imagine how tremendous the shift would be to have a group of ETs
(ideally benevolent) land and introduce themselves.
Many of us have ruminate on the energy and experience of this event for some time, as
we have waited for what seems like a lifetime to witness it.
There are many people claiming to know exactly what this first mass contact experience will
be like.
Some are promoting it as a sort of second coming of Galactic deities or savior figures
while others are attempting to claim that the Earth only has doom in store.
Personally, I don't subscribe to either, as these seem to be more of the ideas of Hollywood
screenwriters than of sensible people.
The experience of first contact will most likely by somewhat of a shock, though a very
positive shock.
I imagine that in some ways we will be cautious, but in many ways we will have a strong sense
of familiarity as though we are meeting those we once knew, but whose faces have changed
over time.
This might be similar to a family reunion.
From the testimonies of whistleblowers such as Corey Goode and William Tompkins, I imagine
the experiences of being around and working with ETs to be much like meeting someone from
a foreign country.
Though there is an initial reaction of shock (as we have been told these beings do not
exist), there is a progressive normalization that takes place.
Once we become used to being around positive beings that look differently than we do, that
is when the true benefit can be realized.
According to these and other whistleblowers, there are numerous beings that look mostly
human, but with subtle differences.
Some of these people look so much like us, that we could not even tell they were ETs
if we walked passed them.
Some of them have even lived and worked with us for thousands of years, and we did not
even notice.
I imagine that during the days of first contact, we will most definitely take notice.
The Futility of Money
It is nice to hear that there is wide-scale financial reform taking place.
This progressive step has been talked about for years now, but in light of the proposal
to simply revamp an old and repressive means of exchange, we must ask the question, "Do
we really need money at all?"
We have all seen the massive amounts of harm and financial manipulation that has taken
place over the last century.
We know of the ills of central banking and the illegitimacy of the fractional reserve
model.
There is also the global slavery we call "employment" which only serves the elitists at the top
of the financial food chain, while dooming the rest of humanity to live in progressive
levels of poverty.
There is no need to prove this to any large extent because it represents the majority
of our life experience.
The past century has brought into question whether or not money needs to exist at all.
The system is reportedly being remade, and new asset-backed currency is being distributed.
However, we must wonder who the new major benefactor is.
Who are the interests distributing this currency and what is their percentage during the process
of exchange?
In my perspective, we cannot truly be free until we realize that there is no need for
money at all.
In essence, money is nothing more than novelty and a tool of middle men.
It is unnecessary, and the longer we hold onto it, the more of our freedom we relinquish
to those who control it.
According to past reports from Kent Dunn, this revaluation represents the last distribution
of money the world will see.
Afterward the plan is to do away with money altogether.
If this is true, that is most definitely something I can look forward to.
This world has much growth and progress to look forward to, and I imagine that the sooner
we choose to free ourselves from the old and outdated systems of control, the faster we
will see that world manifest.
-------------------------------------------
Rep. Walz Asks Agents About Refugee Ban, But Gets No Answers At Airport - Duration: 0:43.I'm Congressman Tim Walz. I represent
Minnesota's 1st congressional district.
It's Monday the 30th of January
about 9:30 a.m. in the morning.
Here at customs and border protection
office asking for some some answers to
questions that constituents of mine have
ranging from who's been detained here
or how the policy's being applied to our
legal permanent residents, our green card holders.
I want to be clear that the
local books working here and the
folks that are providing protection at
the airport, unable to talk to me, unable
to give any statement. But that's not
because they ... they're following orders
from ah.. from Washington. These are folks
that protect us every day. They're doing
their job. The frustration I have with
them being unable answer me or come out
behind the screen, is evident.
But my frustration with...
-------------------------------------------
The Royals 3x09 "O, Farewell, Honest Soldier" Promo (SUB ITA) - Duration: 0:30. For more infomation >> The Royals 3x09 "O, Farewell, Honest Soldier" Promo (SUB ITA) - Duration: 0:30.-------------------------------------------
#4 (ft. Vayne) - Duration: 0:37.*chat*
Now watch me whip...
Now watch me nae nae...
*It was at this moment that he knew he fucked up*
Well...
FUCK!!!
-------------------------------------------
Proper PCR Cleanup before Sanger Sequencing - Seq It Out #12 - Duration: 3:09.Why is a clean template for your Sanger sequencing reaction important?
And what are the ways to get it without losing too much precious samples?
Let's find out today.
The sequencing workflow usually starts with PCR also known as Polymerase Chain Reaction,
where the target fragment is replicated into thousands of copies under a controlled condition
using primers, DNA polymerases and deoxynucleotides.
Before the PCR product is ready for Sanger sequencing, you must do one more thing, a
PCR clean up step.
Because Sanger sequencing is a highly accurate technique for you to read DNA sequence base
by base, it is very important to clean up your reaction mixtures so that those unincorporated
primers and dNTPs won't interfere with your results.
So how do you perform this PCR clean up step and what are your options?
There are several methods for PCR cleanup such as ethanol precipitation, bead or column
based purification, and Enzymatic approaches.
Ethanol precipitation is most cost effective but is also the most labor intensive.
Bead or column based purification can be an effective method for many applications.
However, this route tends to be a bit pricier compared to other options.
This method of purification also requires transferring samples in and out of the column
which can often result in the loss of some of your precious samples so this may not be
the best method for those with limited starting materials.
An enzymatic cleanup approach has a really simple and straightforward workflow.
In fact, it's only one single pipetting step!
It is also relatively affordable compared to the bead or column based purification methods.
Basically, you add an enzyme mix to your finished PCR reaction and let it sit at a certain temperature.
But to understand this method better, let's take a look at our lab book.
One of the enzymes, exonuclease I(one), digests excess primers.
At the same time the other enzyme in the mix, alkaline phosphatase, dephosphorylates nucleotides
in the reaction.
Dephosphorylation means that the enzyme renders the dNTPs from your reaction useless, so that
they don't disturb the sequencing reaction.
This is important because the sequencing reaction has a very specific ratio for its nucleotides
and you don't want to change that ratio.
Now the enzymes have done their work and you don't need them anymore.
You can simply heat up the reaction mix to 80 degrees and this will deactivate the enzymes.
The result is a very clean amplicon that is now ready to go into your sequencing reaction.
You don't need to perform any other steps.
And you didn't lose any of your template either.
You are done!
But I am sure you'll have more questions on PCR cleanup.
Submit your question at thermofisher.com/ask and subscribe to our channel to see more videos
like this.
But remember, when in doubt, just Seq It Out.
-------------------------------------------
Phillips, Craig & Dean Come Now Is The Time To Worship Cover With Unconditional Love Xo Enjoy :D ! - Duration: 2:52.Come, now is the time to worship.
Come, now is the time to give your heart.
Come, just as you are, to worship.
Come, just as you are, before your God.
Come.
One day every tongue will confess You are God.
One day every knee will bow.
Still the greatest treasure remains for those Who gladly choose you now.
Come, now is the time to worship.
Come, now is the time to give your heart.
Oh, come.
Just as you are to worship.
Come just as you are before your God.
Come.
One day every tongue will confess You are God.
One day every knee will bow.
Still the greatest treasure remains for those who gladly choose you now.
[Repeat]
Come, now is the time to worship.
Come, now is the time to give your heart.
Come, just as you are to worship.
Come, just as you are before your God.
Come.
Oh, come.
Oh, come.
Oh, come.
Worship the Lord.
Oh, come.
Come, come, come...
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Sims 4 Vampire Game Pack Overview! Crazy Vampires and Epic Outfits! - Duration: 30:32.REALLY NOT FEELIN UP TO IT RIGHT NOW. SORRY.
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Die Bedeutung der Darmflora/The importance of the gut flora - Duration: 28:12.It's an adventure ...
becoming a mother
and being a mother.
Discover your style ...
Follow your path.
Be inspired by mother and author Sarah Schmid.
Hello, this is Sarah Schmid.
Your commentaries suggested, that I should do yet another video on the topic diet
or more precise on the topic digestion.
Several of you wrote they would never tolerate as much animal protein and other things we eat.
And you are probably right.
I've drawn a little picture here.
This is supposed to be a piece of the mucous membrane of the small intestine:
This pink line...
You can see the intestinal villi here.
They are covered by a protective layer of mucous--which I kept in yellow here.
And you can see all these small blue friendly creatures romping about.
These are bacteria and other microbes
that naturally exist in the intestine and on the mucous membrane there.
They form a real ecosystem
which establishes within the first years of life.
The human body hosts several trillions of these tiny beings.
In the colon there are even more than in the small intestine.
You can wonder what they are all doing there.
They don't make us sick... or do they?
Firstly the function of these bacteria is to modulate, to influence the immune system.
Furthermore they influence emotional behavior and how we deal with stress.
Obviously they generate vitamines... which I sketched in here.
They have a detoxification function
and even protect from harmful bacteria.
Furthermore they supply the mucous membrane with energy
so that it can function well
in absorbing digested food components.
Also the bacteria support digestion
because they help absorbing the food components which I marked green in the picture.
Here you see several green... objects
which are absorbed via the mucous membrane into the blood.
I've put it in a simplified form.
These bacteria have even more tasks which I'll skip.
Anyway healthy digestion doesn't work without properly chewing,
the first enzymes present in the saliva,
gastric acid in the stomach,
bile which is excreted into the duodenum
and enzymes of the pancreas.
Represented by brown and purple drops here.
All if this together makes good digestion
and a person who gets everything he needs to be and remain healthy.
Here I drew another piece of mucous membrane from the small intestine.
You can maybe tell it looks a little bit different.
The membrane is red and inflamed.
The protective layer of mucous is thin
and there are not as many different bacteria as before.
You can assume that now since the bacteria are not as many, they can't perform their tasks as properly as before:
So digestion, supply of the membrane cells,
immune system, detoxification function, vitamin production and protection from harmful bacteria.
Furthermore we now have food components that are not broken down completely.
They cannot be absorbed by the gut.
They accumulate in the intestine and draw putrid bacteria...
bacteria that decompose it.
These emit substances that harm the gut lining.
Represented by the brown clouds here.
If the intestinal lining is slightly inflamed it'll get more permeable
for substances that usually don't pass through.
This way half digested food components get into the blood anyway which don't belong there originally.
There I drew it in: not completely split food parts.
This of course alerts the immune system
and if this happens all the time it'll be in continous alertness.
Then it can happen that the immune system forms antibodies even against harmless substances.
What follows are allergies and autoimmune disorders.
This whole problem is called leaky gut syndrome.
Often also the production of the digestive juices like stomach acid and bile is reduced
which further contributes to an uncomplete digestion of foods.
And so this goes on continuously.
This problem can present in different degrees of severity
from a little stomach ache after meals to
recurring diarrhea alternating with constipation.
Or you can feel really bad and not be able to eat anything.
This problem is especially pronounced in celiac disease for example.
Then you can even see changes under a microscope.
There you'll find an atrophy of the villi.
Here I drew them in normal size. But they can get smaller and smaller
because of the ongoing inflammation that is afflicting them.
How can you tell that something is not right with the gut bacteria?
There are many different symptoms, also depending on age.
With babies it typically shows in colic. They cry a lot, are pretty fuzzy,
difficult to soothe, want to be carried all the time.
The colics occur because in the beginning even breastmilk
--which is normally easy to digest--is not digested well by these babies.
because the gut flora is not optimal.
These babies gain weight rather slowly.
They are no typical chubby babies.
Often they start late on solids.
It can be with 9 or 12 months or even later that they really start eating.
They prefer to stay with breastmilk longer than usual
because breastmilk is easy to digest and all other foods
are hard to digest for these babies.
They feel this and that's why they prefer breastmilk.
Later these kids often eat one-sided,
particularly carbohydrates: noodles, cookies, sweets.
Because it's easiest to digest for them and there are even some other theories on why this is so.
A variety of abnormalities is possible later in life.
Maybe starting at early childhood, I would say.
Food intolerances with bloating, stomach ache, diarrhea,
the irritable bowel syndrome.
Neurodermatitis, allergies, asthma as expression for the ongoing chronic inflammation that takes place
and the overly alert immune system.
Psychological disorders can accompany this
like ADHD, Asperger's, depression.
In later life other diseases as expression of the dysregulation of the immune system can be added
like cancer or autoimmune disorders.
In pregnancy as a typical sensitive period this can show in extreme or prolonged morning sickness.
The body seems to know what it can't digest well
and tries to protect itself and the baby from the immune reaction to incomplete digested food components in the blood.
Another problem that is on the rise in the western world is prematury.
Despite all efforts and prenatal care, doctors are unable to stop it.
Often it's caused by an ascending infection
but an infection can only ascend when the natural bacterial flora is disturbed.
Bacterial flora does not only relate to the gut but to the whole person
and even though there are different kinds of bacteria in different places
the overall bacterial population of the body gets influenced by the body these bacteria live on.
It's a whole.
You cannot view the gut isolated from the vagina, the mouth or the skin.
If it doesn't work well on one end,
then it probably won't work so well on the other end either.
In pregnancy it can get noticable more easily.
Urinary tract infection and fungal infection range in the same category.
All these bacteria and fungi that you don't want there can spread much easier, if the natural bacterial population is not well intact.
You can wonder where these problems come from today.
Why is our gut flora disturbed?
If you think back not long ago
our grandparents in their younger years--or great-grandparents, depending on how old you are--
they harldy knew about the diseases I just talked about.
There maybe was one with asthma or hay fever
but it was not so that every second had something like this.
And if you look back even further it becomes even more uncommon.
So it only started and increased in the 20th century.
And in particular they ate all those foods that we like to blame to make us sick today.
Something has changed
because back then nobody was eating vegan for example.
Even the word "vegan" itself was unknown
There were some health-conscious people who went vegetarian--at least for a while.
But that was it.
So it can't be the foods fault alone that most people don't tolerate something or have allergies.
I like to ask the question: What was different back then compared to today?
And there you can find pretty much. Lots of things have changed over the last century.
My grandparents where--at least half of them--still born at home.
My grandparents on my father's side lived on the countryside and were born at home.
On my mother's side they lived in the city, everything there was more modern already and they were born in the hospital.
Anyway the bacterial milieu in the hospital is different.
The bacteria the baby comes in contact with in the first days and hours of life
shape the bacterial flora he will have for his whole life.
Also there where hardly any C-sections back then.
A C-section provides another bacterial flora than a vaginal birth.
And our grand- und great-grandparents virtually grew up without antibiotics.
Furthermore the milk they drank--and milk they did drink--
was delivered fresh and raw and also consumed this way.
Even my mother still has memories of fresh milk waiting at the door every day.
Later on they boiled the milk because of fear for tuberculosis
but still it was a different handling of the milk than today.
Also the children were not vaccinated against everything you can think of.
There was the smallpox vaccination. And that's it.
At least for our grandparents.
Then there was no fast food and no McDonalds.
The mothers were at home.
They were not supposed to go to work like today
but they were allowed to take care of their kids
without being vilified by society for this.
They cooked fresh from main ingredients
because ready prepared foods didn't exist yet.
Probably they also consumed less sugar than today
where sugar is added to virtually everything
which is not a basic food like vegetables or so.
And the kids were playing outdoor and in the dirt a lot.
This was not just dirt, almost everybody had domestic animals.
So they got in contact with a variety of germs, that our kids don't meet even if they play in the dirt.
What can we do to keep a healthy gut despite modern living?
Of course it's difficult to shake off the bacterial imprint
you got in your mother's womb and after birth.
So if you have allergies or food intolerances from birth or childhood on
then complete healing is rather impossible.
But it's possible to achieve a clear improvement
so that you can tolerate all or most foods--at least in certain amounts.
If these problems started later in life they seem easier to overcome.
But the goal should be to heal the gut
and to get the chronic inflammation out.
How do you do it? How can it be done?
Of course the obvious is--and that's what most people try or do--to avoid those foods that are not tolerated well,
are not digested well and maintain the imbalance.
These are often lactose and other di- and polysaccharides,
different proteins, most often from dairy and grain.
And foods that contain phytic acid.
Grains fall in this category but even beans and nuts.
You don't have to avoid all this together
but with these you need to be careful with preparation and test out what you tolerate.
It's interesting to note that many people who don't tolerate milk,
don't tolerate store bought milk which is pasteurised, homogenised and ultrafiltered.
But often they tolerate raw milk.
And if someone doesn't tolerate raw milk
often he can still eat soured raw milk.
And sometimes small amounts are tolerated but not large ones.
So you can test out what you tolerate and what not.
It's not an all-or-nothing principle
but often the body can handle smaller or larger amount depending on the constitution.
Initially it's a good idea to avoid everything that's not tolerated completely
so that the gut can heal and the inflammation disappear.
After that it can absorb nutritions better
and these half digested foods don't get into the blood anymore.
All the inflammation has to get out of there.
This also explains why symptoms can be reduced by a vegan diet.
Because there you avoid what a not so healthy gut cannot digest.
In the long run of course protein elements, vitamins and micronutrients are lacking
that are not or not sufficiently found in plant food.
But short-term you can achieve a significant improvement without long-term consequences.
But to deal with it in the long run, there is a second step:
Adding foods that help the gut to heal.
This means above all many good bacteria.
Sauerkraut for example,
fermented vegetables...
You can make a lot at home.
Instructions you can find on the internet.
Proteins you eat should be easy to digest.
Animal protein is easiest to digest, easier than plant protein
and it's easier to digest if eaten raw, marinated or fermented
or just so that it tastes good.
not overcooked or... What lies heavy in the stomach you should better avoid.
Bone broth is an important element
that contains a lot that helps the gut to regenerate.
It has minerals, gelatine and many other things that help repair the damaged gut lining.
And of course very important the fatsoluble vitamins D and A.
The classic cod liver oil I already talked about in previous videos.
It's also important to avoid industriell produced fats
who can contribute to an inflammatory process because of their trans fat content.
Instead grass-fed butter, coconut oil... these contain good fats you'll benefit from.
If the gut has healed you can slowly start
adding foods that were not tolerated before.
Best one after the other. So in case you still don't tolerate it
you know what it is and can leave it out again.
But in certain amounts these should be tolerated again.
You see that in principle this is going toward traditional diet, as I presented in my last videos on diet.
For those who want to read further into it:
The approach I just described is explained more detailed in this book:
The GAPS-diet. How gut and mind influence each other.
(The English title is: "Gut and Psychology Syndrome")
To find traditional recipes is not so easy.
I'm providing some on my (German) website for you.
On fermentation there are also books available in German.
For those who speak English
I can recommend this book:
Nourishing Traditions by Sally Fallon.
This book contains lots of traditional recipes,
how to lacto-ferment all kind of foods
and a lot of knowledge about a healthy traditionell diet.
If you are looking for old recipes it can help to
look into old cookbooks.
There many things are still done the traditional way.
What can you do to get children with a healthy gut?
The best you can do is to become as healthy as possible yourself before you even get pregnant.
Unfortunately conventional medicine views bacteria still mostly as harmful.
It has started to shift, though,
but the focus still rests heavily on the harmful side
and not the benefit these little creatures bring.
Unfortunately this is also reflecting in the dietary recommendations for pregnancy
where you are supposed to eat only what is cooked dead.
Main thing: not raw and no bacteria
because they could make you sick.
Instead many good bacteria would be so important in pregnancy
to prevent the whole asthma, allergy and food intolerance problem.
Studies show for example, that drinking raw milk in pregnancy
lowers asthma rates.
But they don't recommend it because of their fear of Listeria
instead of presenting the information in a balanced way.
What you can do else is give birth at home.
At home you have your familiar bacteria
that the immune system of the mother is used to
and the baby via the mother, too.
At least you should opt for a vaginal birth
because then the baby gets in contact with the mother's bacteria first.
Breastfeeding is another important aspect
and of course dealing naturally with disease
where you should avoid medication and especially antibiotics as much as you can.
Better being cautious with vaccinations of course, too.
Best to vaccinate after the first year of life, if you want to vaccinate.
And what is proven as well to be preventive
is regular contact with animals.
Getting a dog for example can prevent allergies
or to visit a farm regularly.
What is it like in our family?
We have not been spared by this modern plague either.
I'm a child from the GDR (German Democratic Republic, one of the two German states before Germany was reunited in 1990)
and happy and healthy.
I don't have any allergies or intolerances.
I can eat everything and am very thankful for that. (It is known that allergy rates differed significantly between East- and West-Germany until the wall came down.)
My husband as a typical Wessi (that's what we call those from West-Germany)
--a side blow to the Wessis--
is very allergic since childhood.
He has something like irritable bowel syndrome I would say.
And it is also limiting to is mental health.
But he is not open to a holistic approach.
He has his conventional medicin which suppresses his symptomes
and with this he is more or less content.
Our oldest was a colic-baby unprecedented
cried a lot, slept very poorly,
gained weight rather moderate in the beginning.
With 9 months she gradually started eating.
Shortly before her 7th birthday she developped allergic symptoms for the first time,
running nose, congested nose during the winter months.
So probably dust mites.
However until now she refuses to have an allergy test.
So I can't tell you more.
When I was pregnant with her I had no idea about all I just told you.
My diet was mediocre,
quite a lot of sugar
and the canteen food in the hospital.
My last rounds of antibiotics had not been so long ago
because of a severe chronic sinusitis.
In this pregnancy I had no antibiotics.
But when she was 3 months old, I took antibiotics one more time.
It has been the last time to this day.
From this she got diarrhea.
I believe it harmed her and pushed her long-term further into the allergic corner.
At the moment she has a chronically more or less congested nose.
And with common colds she is affected the most.
But she recovers quite quickly,
so the level of suffering is not especially high.
I would like it if she tried the GAPS-diet
but I guess her level of suffering is not high enough for this
or she needs to get older first.
But she avoids milk products on her own initiative
so I think she tolerates it poorly.
I rely on her feeling for her body there.
This is the best I think.
She also refuses any medication.
My husband offered her medication for symptom relief several times
but she doesn't want it.
And I'm not unhappy about it
because I don't think it would help improve the imbalance.
So we are dealing with it without medication so far.
Our 2nd child has no allergies or intolerances,
but he would probably get an ADHD diagnosis.
When I was pregnant with him I started drinking raw milk.
Prior to our 3rd child I got through my sugar withdrawel.
From the 4th child on we adapted a diet after Weston Price.
Except for the first child all children were easy babies.
No excessive crying, no colics. Very enjoyable.
As different as day and night.
And except for the first two they all were easy toddlers.
I still can't say this about our youngest, of course.
He is only 4,5 months now.
But so far he is a very balanced baby, as you also may have noticed.
And none of our children has allergies or intolerances
except for the oldest.
Don't get me wrong,
I think our kids are all very adorable and fantastic people.
But if you've had or have a high-need baby or toddler
you know how hard this can be and how it pushes your limits
So I was very happy when I discovered that this is not just fate dropping on my head
but I can influence it.
As you see, it is possible to have
healthy children despite hereditary predispositon.
And that's my wish: That you and your children become, are and remain healthy in your/their gut.
Life is just more enjoyable then.
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Hyundai Tucson 2.0i Dynamic - Duration: 1:13. For more infomation >> Hyundai Tucson 2.0i Dynamic - Duration: 1:13.-------------------------------------------
Citroën Xsara Picasso 1.8i-16V Différence - Duration: 0:52. For more infomation >> Citroën Xsara Picasso 1.8i-16V Différence - Duration: 0:52.-------------------------------------------
winnie the pooh dancing to pitbull but it is recorded with a cheap screen recorder - Duration: 0:26.this video is a joke
why just why
i'm serious
end me
bleach
why did i do this
i need to get bleach bye
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PEPPERMINT MOCHA FRAPPE DIY - Duration: 6:09.Sorry for the bad editing I'm new at this
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Beer, What is it? - Duration: 0:37.Beer, what is it?
Beer, is actually different kinds of fermented wheat,
Different wheat equals different beer.
You can find beer anywhere! This is at Disney land!
This was a Jada Quinn Production film. Thanks for watching!
More on this topic later!
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Three Little Kittens For more infomation >> Three Little Kittens-------------------------------------------
Wheels On The Bus Rhyme For more infomation >> Wheels On The Bus Rhyme-------------------------------------------
The Royals 3x09 "O, Farewell, Honest Soldier" Promo (SUB ITA) - Duration: 0:30. For more infomation >> The Royals 3x09 "O, Farewell, Honest Soldier" Promo (SUB ITA) - Duration: 0:30.-------------------------------------------
Right? Wrong? Irrelevant. - Duration: 0:15.What do you learn at your second breakdown?
It's the golden rule I am taking from now on, which is:
"For me it's irrelevant if I am right or wrong anymore."
I'm going to do what I want
and that's it.
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Protests Continue Monday At O'Hare In Response To Travel Ban - Duration: 2:00.ATTORNEY GENERAL SAY THAT IS HE
IS SUING THE GOVERNMENT.
LET'S GET RIGHT TO LAUREN WHO
IS LIVE AT O'HAIR AIRPORT.
LAUREN?
Reporter: WELCOME PAIRED TO
SATURDAY AND SUNDAY, IT'S A
LITTLE BIT QUIETER.
THERE ARE
PROTERSERS TO THE RIGHT.
I WANT
TO SHOW THE ATTORNEYS HERE RIGHT
NOW.
THEY TELL ME THAT NO ONE
IS FORMERLY BEING DETAINED AT
THIS AIRPORT RIGHT NOW.
EVERY
CREEK OF PEEK BY HER LOVED ONE
BEING TIED UP IN CUSTOMS.
JUST DON'T MIND.
THEY ASK
YOU AGAIN AND AGAIN AND AGAIN
JUST KEEP ON TELLING THEM WHY
YOU'RE HERE.
Reporter: AN HOUR LONG WAIT
ASIDE AND NO MAJOR PROBLEMS FOR
THE FAMILY.
DID THEY ASK YOU
ANY ADDITIONAL QUESTIONS?
NO.
NO.
Reporter: THEIR HOMELAND IS
NOT EXECUTIVE.
THEY HAVE A RIGHT TO CHECK
EVERYONE, THAT'S OKAY.
Reporter: SHE THINKS THAT AN
OUT RIGHT BAN IS TOO FAR AND
OTHERS FEAR THAT IT'S GOING GO
TOO FAR.
ARE YOU NOT PLANNING A
VACATION BACK TO MEXICO?
NO.
NO.
I DON'T KNOW WHAT
HAPPENS WHEN I RETURN TO THE
UNITED STATES.
Reporter: SOME TRAVELLERS ON
THE LIST ARE TAKING ADVANTAGE OF
A FEDERAL JUDGES STAY.
I HAD TO COME BACK.
I
THOUGHT YEAH, I'M NOT GOING TO
ENJOY THE REST OF THE TRIP
BECAUSE I DON'T KNOW WHAT WILL
HAPPEN.
Reporter: NOW BACK OUT HERE
LIVE THESE LAWYERS SAY THAT
THEY'RE STANDING BY TO HELP AT
O'HAIR AND SCHEDULE ON SHIFTS
THROUGH FRIDAY.
RESPONSE FROM
THE AREA ATTORNEYS IS SO STRONG
THAT ORGANIZERS TWEETED OUT THAT
THEY DO NOT NEED ANYMORE
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Snapchat Story: Chinese New Year 2017 in Chinatown, London (Year of the Rooster) - Duration: 2:11.Hello to all the followers of @codigonuevo
My name is Oriol Salvador, I'm in London and I'll bring you to Chinatown to see how they celebrate
Chinese New Year.
Chinese New Year or Spring Festival started last Friday and goes on until February 22nd.
Celebrations in London's Chinatown are mainly TODAY.
What is he doing?, may you ask. Bringing good luck to stores and restaurants in the new year, they told me.
Don't be fooled by souvenirs stores, 'maneki neko' (this cat) is not Chinese,
it's Japanese.
Later we'll go back to Chinatown but now we go to Trafalgar Square where Chinese community
is celebrating the New Year with shows and food stalls.
Access to Trafalgar Square was closed, but we're in now!
Visiting Hong Kong's skyscrappers on a VR experience.
"How can they jump so high?!"
"Are you hungry?"
Find out what animal on Chinese zodiac are you according to your birth date. I'm a dragon.
"Happy Chinese New Year everyone. We are celebrating the Year of the Rooster,
we celebrate everyone born in the year of the Rooster and hope you have lots of prosperity
and good luck in the new year.
Everyone wants to take a picture with this man,
he is the God of fortune and gives away red envelopes
with money inside, to attract
wealth on the new year.
That's it from me from the Chinese New Year in London's Chinatown.
It's being a pleasure to share it with you.
You can find me in other social media as @oriolsalvador
Oh! If you also want to be an ambassador for @codigonuevo Snapchat, get in touch NOW
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